Document Detail

Culture of cells from zebrafish (Brachydanio rerio) blastula-stage embryos.
MedLine Citation:
PMID:  7765109     Owner:  NLM     Status:  MEDLINE    
The zebrafish has become a popular model for studies of vertebrate development and toxicology. However, in vitro approaches utilizing this organism have not been fully exploited due to the absence of suitable cell culture systems. Previously, we developed methods for the culture of cells derived from zebrafish blastula-stage embryos. One of these cultures, ZEM-2, was derived in a complex medium containing trout embryo extract, trout serum and medium conditioned by buffalo rat liver cells. In this study we describe a zebrafish embryo cell line, ZEM-2A, derived from ZEM-2 following selection for growth in a simplified medium. Optimal growth of ZEM-2A cells is attained in nutrient medium supplemented with 5% fetal bovine serum.
C Ghosh; P Collodi
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Cytotechnology     Volume:  14     ISSN:  0920-9069     ISO Abbreviation:  Cytotechnology     Publication Date:  1994  
Date Detail:
Created Date:  1994-09-22     Completed Date:  1994-09-22     Revised Date:  2008-10-09    
Medline Journal Info:
Nlm Unique ID:  8807027     Medline TA:  Cytotechnology     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  21-6     Citation Subset:  B    
Department of Animal Sciences, Purdue University, West Lafayette, Indiana 47907.
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MeSH Terms
Blastocyst / cytology*
Cell Division
Cells, Cultured*
Culture Media
Zebrafish / embryology*
Reg. No./Substance:
0/Culture Media

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