Document Detail


Culture of adult human islet preparations with hepatocyte growth factor and 804G matrix is mitogenic for duct cells but not for beta-cells.
MedLine Citation:
PMID:  9421388     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It has recently been reported that human adult beta-cells proliferate during culture on an extracellular matrix prepared from rat 804G cells and in the presence of hepatocyte growth factor (HGF). The present study compares the mitogenic effect of this condition on human beta-cells and on neighboring non-endocrine duct cells. Islet cell-enriched fractions were prepared from adult human organ donors and cultured in suspension or on 804G matrix, with or without HGF. The combination of 804G matrix and HGF increased the number of 5-bromo-2'-deoxyuridine-positive (BrdU+) cells within 48 h reaching a maximum after 4 days. In sections, virtually all BrdU+ cells were negative for insulin or glucagon and for preproinsulin mRNA but expressed the ductal cell markers cytokeratin 19 and 7, carbonic anhydrase-II, and carbohydrate antigen 19-9. After 4 days of culture, the cytokeratin 19+ ductal cells exhibited a BrdU-labeling index of 30% (P < 0.01 vs. 2% without HGF and matrix), whereas <0.1% of insulin-positive and <1% of glucagon-positive cells were labeled. Formation of bilayers with ductal cells covering the endocrine cells may cause erroneous interpretation on double positivity in unsectioned tissue. It is concluded that culture of human islet cell preparations with HGF and 804G matrix stimulates the proliferation of the duct cells but not of the underlying beta-cells.
Authors:
V H Lefebvre; T Otonkoski; J Ustinov; M A Huotari; D G Pipeleers; L Bouwens
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Diabetes     Volume:  47     ISSN:  0012-1797     ISO Abbreviation:  Diabetes     Publication Date:  1998 Jan 
Date Detail:
Created Date:  1998-01-22     Completed Date:  1998-01-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372763     Medline TA:  Diabetes     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  134-7     Citation Subset:  AIM; IM    
Affiliation:
Diabetes Research Center, Vrije Universiteit Brussel, Brussels, Belgium. velefeb@mebo.vub.ac.be
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MeSH Terms
Descriptor/Qualifier:
Adult
Animals
Bromodeoxyuridine
CA-19-9 Antigen / analysis
Carbonic Anhydrases / analysis
Cell Division / drug effects,  physiology
Cells, Cultured
Extracellular Matrix / physiology*
Glucagon / analysis
Hepatocyte Growth Factor / pharmacology*,  physiology
Humans
Insulin / analysis
Islets of Langerhans / chemistry,  cytology*,  metabolism
Keratins / analysis
Middle Aged
Pancreatic Ducts / chemistry,  cytology*,  metabolism
Proinsulin / analysis,  genetics
Protein Precursors / analysis,  genetics
RNA, Messenger / analysis,  genetics
Rats
Recombinant Proteins / pharmacology
Time Factors
Chemical
Reg. No./Substance:
0/CA-19-9 Antigen; 0/Protein Precursors; 0/RNA, Messenger; 0/Recombinant Proteins; 11061-68-0/Insulin; 59-14-3/Bromodeoxyuridine; 61116-24-3/preproinsulin; 67256-21-7/Hepatocyte Growth Factor; 68238-35-7/Keratins; 9007-92-5/Glucagon; 9035-68-1/Proinsulin; EC 4.2.1.1/Carbonic Anhydrases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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