Document Detail


Culture of Schistosoma haematobium in vivo and in vitro.
MedLine Citation:
PMID:  1267504     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The maximum rate of development of Schistosoma haematobium in the hamster was determined by examination of the most advanced worms recovered at short intervals throughout the course of development. In culture S. haematobium developed at the same rate as in the hamster up to day 31 when pairing first occurs and male worms produce some spermatozoa. In vitro males formed some spermatozoa but pairing did not take place and, probably for this reason, females did not complete sexual maturation as occurs in the host between days 57-65. Somatic growth continued in vitro and at 70 days male worms had achieved almost the same length as in the hamster at this time. The culture medium, previously used for S. mansoni, consisted of equal volumes of serum and Earle's balanced saline with a final concentration of 0.25% lactalbumin hydrolysate, 100 units/ml penicillin, 100 mug/ml streptomycin and 1% rbc. The best culture results were obtained with one particular human serum; seven other human sera gave a wide range of growth support. The samples of baboon, rhesus monkey or foetal calf sera tested provided little or no growth support but prolonged survival was possible in all the sera.
Authors:
M Smith; J A Clegg; G Webbe
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Annals of tropical medicine and parasitology     Volume:  70     ISSN:  0003-4983     ISO Abbreviation:  Ann Trop Med Parasitol     Publication Date:  1976 Mar 
Date Detail:
Created Date:  1976-07-06     Completed Date:  1976-07-06     Revised Date:  2009-05-18    
Medline Journal Info:
Nlm Unique ID:  2985178R     Medline TA:  Ann Trop Med Parasitol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  101-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cricetinae
Female
Liver / parasitology
Lung / parasitology
Male
Ovum / cytology
Schistosoma haematobium / cytology,  growth & development*,  physiology
Schistosomiasis / parasitology
Skin / parasitology
Spermatozoa / cytology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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