Document Detail


Culture of Blomia tropicalis and IgE immunoblot characterization of its allergenicity.
MedLine Citation:
PMID:  10697258     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Blomia tropicalis is an important triggering factor for allergic diseases such as asthma, rhinitis and atopic dermatitis in tropical and subtropical regions, which climate favours the growth of this species. Our previous mite fauna study revealed that Blomia tropicalis is the most dominant species present in Singapore house dust The main objective of this study is to establish a mass culture of Blomia tropicalis for further characterization of the antigenic and molecular properties of this mite. Approximately one gram of mites could be obtained for every 300-gram of culture medium by culturing under natural condition with a mean annual temperature of 30 degrees C and a mean relative humidity of 80%, and harvested by modified Tullgren funnel. Allergen characterization by IgE immunoblot analysis with crude mite extracts showed some IgE reactivity differences between Blomia tropicalis mite extract from Singapore and Colombia. The possible reasons for these findings are the quality and source of the mite protein extracts used, or selective differences in the population under evaluation. Further, the atopic sera tested showed differences in the pattern and Intensity of IgE immunoblot reactivity to crude extracts of Blomia tropicalis and Dermatophagoides pteronyssinus, the other highly prevalent mite in Singapore. These data support the existence of species-specific allergens. In conclusion, we have been successful in setting up B. tropicalis mass cultures and have prepared extracts of high allergenicity.
Authors:
F C Yi; F T Chew; S Jimenez; K Y Chua; B W Lee
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Asian Pacific journal of allergy and immunology / launched by the Allergy and Immunology Society of Thailand     Volume:  17     ISSN:  0125-877X     ISO Abbreviation:  Asian Pac. J. Allergy Immunol.     Publication Date:  1999 Sep 
Date Detail:
Created Date:  2000-04-04     Completed Date:  2000-04-04     Revised Date:  2006-04-19    
Medline Journal Info:
Nlm Unique ID:  8402034     Medline TA:  Asian Pac J Allergy Immunol     Country:  THAILAND    
Other Details:
Languages:  eng     Pagination:  189-94     Citation Subset:  IM    
Affiliation:
Department of Paediatrics, National University of Singapore, Singapore.
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MeSH Terms
Descriptor/Qualifier:
Allergens / immunology*
Animals
Dust
Electrophoresis, Polyacrylamide Gel
Humans
Immunoblotting*
Immunoglobulin E / immunology*
Microscopy, Electron, Scanning
Mites / growth & development*,  immunology*
Chemical
Reg. No./Substance:
0/Allergens; 0/Dust; 37341-29-0/Immunoglobulin E

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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