Document Detail

Cultivation on solid media of spirochetes in subgingival plaque from advanced marginal periodontitis in humans.
MedLine Citation:
PMID:  6593809     Owner:  NLM     Status:  MEDLINE    
Six solid substrates were compared for suitability to support growth of spirochetes from 18 subgingival plaque samples in patients with advanced marginal periodontitis. The following substrates with rabbit serum were tested: BHIA-SC + blood, RGCA-SC + blood, GM-1 +/- blood and MGM-1 +/- blood. All media contained rifampicin and polymyxin B. After incubation in a glovebox (70% N2, 20% H2, 10% CO2) for 14 days spirochete colonies were semiquantitated and the colony morphologies described. The spirochetes in six of the primary samples and in the related colonies after cultivation were identified ultrastructurally by number of endoflagella. The results showed that the colony morphologies were more related to the substrate used than to the various types of spirochetes, and that individual colonies contained different types of spirochetes. The substrates MGM-1, RGCA-SC, and BHIA-SC, all with blood, showed the highest recoveries of about 5%. Spirochetes with less than four endoflagella grew on all substrates, but the MGM-1 + blood and RGCA-SC + blood media seemed the most versatile as they showed growth of all types of spirochetes observed in the primary samples.
N E Fiehn; J Westergaard
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Scandinavian journal of dental research     Volume:  92     ISSN:  0029-845X     ISO Abbreviation:  Scand J Dent Res     Publication Date:  1984 Oct 
Date Detail:
Created Date:  1984-12-12     Completed Date:  1984-12-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0270023     Medline TA:  Scand J Dent Res     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  426-35     Citation Subset:  D; IM    
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MeSH Terms
Culture Media / metabolism
Dental Plaque / microbiology*
Gingival Pocket / microbiology
Microscopy, Electron
Middle Aged
Periodontitis / microbiology*
Spirochaetales / growth & development*,  isolation & purification,  ultrastructure
Reg. No./Substance:
0/Culture Media

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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