Document Detail


Cultivation and maintenance of Sphaerophorus necrophorus. I. An anaerobic medium for aerobic incubation.
MedLine Citation:
PMID:  4272964     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Out of 185 media formulated and studied for the growth of Sphaerophorus necrophorus, ten were selected for intensive investigation. Their ability to promote growth was compared photometrically. Medium 156 made up of Brewer's thioglycollate broth, yeast extract, L-cystine, and ascorbic acid, was found to yield a very heavy growth. The viability of S. necrophorus was maintained indefinitely by weekly subcultures. When not subcultured, all isolates remained viable for fifteen weeks and some isolates for 30 weeks at 37 degrees C.Twenty-nine isolates were tested. No variation in maximum growth was noticed. Medium 156 is an optimum medium for S. necrophorus. It can be incubated aerobically without interfering with maximum growth. The medium does not deteriorate when stored for at least 12 weeks at room temperature.
Authors:
P C Simon
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Canadian journal of comparative medicine. Revue canadienne de médecine comparée     Volume:  38     ISSN:  0008-4050     ISO Abbreviation:  Can. J. Comp. Med.     Publication Date:  1974 Jan 
Date Detail:
Created Date:  1974-04-03     Completed Date:  1974-04-03     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0151747     Medline TA:  Can J Comp Med     Country:  CANADA    
Other Details:
Languages:  eng     Pagination:  94-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Aerobiosis
Anaerobiosis
Culture Media
Fusobacterium / growth & development*
Hydrogen-Ion Concentration
Temperature
Chemical
Reg. No./Substance:
0/Culture Media
Comments/Corrections

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