Document Detail


Cultivation of lacrimal gland acinar cells in a microgravity environment.
MedLine Citation:
PMID:  19416938     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: A rotary cell-culture system (RCCS) allows the creation of a microgravity environment of low shear force, high-mass transfer and three-dimensional cell culture of various cell types. The aim of the study was to evaluate the growth pattern and the secretory function of rabbit lacrimal gland acinar cells in a microgravity environment using an RCCS. METHODS: Lacrimal gland acinar cells from male New Zealand White rabbits were isolated and cultured in an RCCS up to 28 days. Cells were analysed by light and electron microscopy, and apoptosis was assessed by the TUNEL assay at days 7, 14, 21 and 28. Secretory function was tested by measuring the beta-hexosaminidase activity. RESULTS: After 7 days of culture, spheroidal aggregates were found inside the RCCS. The spheroids consisted of acinus-like cell conglomerates. Apoptotic centres inside the spheroids were observed at all time points by means of the TUNEL assay. Evaluation of the secretory function revealed beta-hexosaminidase release after carbachol stimulation which decreased over the culture period. CONCLUSION: A simulated microgravity environment promotes the development of three-dimensional cell spheroids containing viable acinar cells up to 28 days. Due to the evolving central apoptosis, it is unlikely that such simple three-dimensional cell communities can serve as tissue equivalents for clinical transplantation, but they promise opportunities for further applications in basic and applied cell research on lacrimal gland cells.
Authors:
S Schrader; C Kremling; M Klinger; H Laqua; G Geerling
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-05-04
Journal Detail:
Title:  The British journal of ophthalmology     Volume:  93     ISSN:  1468-2079     ISO Abbreviation:  Br J Ophthalmol     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-07-27     Completed Date:  2009-09-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0421041     Medline TA:  Br J Ophthalmol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1121-5     Citation Subset:  IM    
Affiliation:
Department of Ophthalmology, University of Lübeck, Lübeck, Germany. mail@stefanschrader.de
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis
Carbachol / pharmacology
Cell Culture Techniques / methods
Cell Division
DNA Fragmentation
In Situ Nick-End Labeling / methods
Lacrimal Apparatus / cytology*,  drug effects,  enzymology,  ultrastructure
Male
Microscopy, Electron
Rabbits
Spheroids, Cellular / ultrastructure
Weightlessness Simulation*
beta-N-Acetylhexosaminidases / metabolism
Chemical
Reg. No./Substance:
51-83-2/Carbachol; EC 3.2.1.52/beta-N-Acetylhexosaminidases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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