| Cultivation-independent assessment of the bacterial diversity of breast milk among healthy women. | |
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MedLine Citation:
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PMID: 17224259 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Breast milk has been shown to be an excellent and continuous source of commensal and potentially probiotic bacteria to the infant gut. Our aim was to evaluate the dominant bacteria existing in breast milk of healthy women and the potential role of transit through the vagina in the acquisition of breast milk microbiota using the 16S rRNA amplified gene approach. Samples of breast milk were aseptically collected, at day 7 after delivery, from five mothers whose neonates were born by vaginal delivery and from five others who had had their babies by programmed elective cesarean section. All mothers were healthy, had a full-term pregnancy and breastfed their infants. DNA extracted from biological samples was used as a template for PCR amplification of 16S rRNA gene sequences with universal bacterial primers; then the PCR products were analyzed by denaturing gradient gel electrophoresis (DGGE); finally, clone libraries of 16S rRNA gene sequences from 4 mothers (2 from each group) were constructed. PCR DGGE patterns and clone libraries suggest that each woman had a specific bacterial pattern in her breast milk, and confirm, at the molecular level, that breast milk of healthy women is a source of commensal bacteria to the infant gut. They also reinforce recent molecular studies which have shown that lactic acid bacteria colonization is not significantly related to the delivery method. |
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Authors:
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Rocío Martín; Hans G H J Heilig; Erwin G Zoetendal; Esther Jiménez; Leónides Fernández; Hauke Smidt; Juan M Rodríguez |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2006-12-19 |
Journal Detail:
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Title: Research in microbiology Volume: 158 ISSN: 0923-2508 ISO Abbreviation: Res. Microbiol. Publication Date: 2007 Jan-Feb |
Date Detail:
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Created Date: 2007-02-13 Completed Date: 2007-06-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8907468 Medline TA: Res Microbiol Country: France |
Other Details:
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Languages: eng Pagination: 31-7 Citation Subset: IM |
Affiliation:
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Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Universidad Complutense de Madrid, 28040 Madrid, Spain. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AM157417; AM157418; AM157419; AM157420; AM157421; AM157422; AM157423; AM157424; AM157425; AM157426; AM157427; AM157428; AM157429; AM157430; AM157431; AM157432; AM157433; AM157434; AM157435; AM157436; AM157437; AM157438; AM157439; AM157440; AM157441; AM157442; AM157443; AM157444; AM157445; AM157446; AM157447; AM157448; AM157449; AM157450; AM157451; AM157452 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacteria
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classification*,
genetics,
isolation & purification Cesarean Section Electrophoresis, Gel, Two-Dimensional Female Gene Library Humans Live Birth Milk, Human / microbiology* Molecular Sequence Data Polymerase Chain Reaction RNA, Bacterial / analysis, genetics RNA, Ribosomal, 16S / analysis, genetics Species Specificity |
| Chemical | |
Reg. No./Substance:
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0/RNA, Bacterial; 0/RNA, Ribosomal, 16S |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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