Document Detail


Cultivation-independent assessment of the bacterial diversity of breast milk among healthy women.
MedLine Citation:
PMID:  17224259     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Breast milk has been shown to be an excellent and continuous source of commensal and potentially probiotic bacteria to the infant gut. Our aim was to evaluate the dominant bacteria existing in breast milk of healthy women and the potential role of transit through the vagina in the acquisition of breast milk microbiota using the 16S rRNA amplified gene approach. Samples of breast milk were aseptically collected, at day 7 after delivery, from five mothers whose neonates were born by vaginal delivery and from five others who had had their babies by programmed elective cesarean section. All mothers were healthy, had a full-term pregnancy and breastfed their infants. DNA extracted from biological samples was used as a template for PCR amplification of 16S rRNA gene sequences with universal bacterial primers; then the PCR products were analyzed by denaturing gradient gel electrophoresis (DGGE); finally, clone libraries of 16S rRNA gene sequences from 4 mothers (2 from each group) were constructed. PCR DGGE patterns and clone libraries suggest that each woman had a specific bacterial pattern in her breast milk, and confirm, at the molecular level, that breast milk of healthy women is a source of commensal bacteria to the infant gut. They also reinforce recent molecular studies which have shown that lactic acid bacteria colonization is not significantly related to the delivery method.
Authors:
Rocío Martín; Hans G H J Heilig; Erwin G Zoetendal; Esther Jiménez; Leónides Fernández; Hauke Smidt; Juan M Rodríguez
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-12-19
Journal Detail:
Title:  Research in microbiology     Volume:  158     ISSN:  0923-2508     ISO Abbreviation:  Res. Microbiol.     Publication Date:    2007 Jan-Feb
Date Detail:
Created Date:  2007-02-13     Completed Date:  2007-06-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8907468     Medline TA:  Res Microbiol     Country:  France    
Other Details:
Languages:  eng     Pagination:  31-7     Citation Subset:  IM    
Affiliation:
Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Universidad Complutense de Madrid, 28040 Madrid, Spain.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AM157417;  AM157418;  AM157419;  AM157420;  AM157421;  AM157422;  AM157423;  AM157424;  AM157425;  AM157426;  AM157427;  AM157428;  AM157429;  AM157430;  AM157431;  AM157432;  AM157433;  AM157434;  AM157435;  AM157436;  AM157437;  AM157438;  AM157439;  AM157440;  AM157441;  AM157442;  AM157443;  AM157444;  AM157445;  AM157446;  AM157447;  AM157448;  AM157449;  AM157450;  AM157451;  AM157452
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MeSH Terms
Descriptor/Qualifier:
Bacteria / classification*,  genetics,  isolation & purification
Cesarean Section
Electrophoresis, Gel, Two-Dimensional
Female
Gene Library
Humans
Live Birth
Milk, Human / microbiology*
Molecular Sequence Data
Polymerase Chain Reaction
RNA, Bacterial / analysis,  genetics
RNA, Ribosomal, 16S / analysis,  genetics
Species Specificity
Chemical
Reg. No./Substance:
0/RNA, Bacterial; 0/RNA, Ribosomal, 16S

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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