Document Detail

Cultivation of hamster kidney cells in a dynamic cell culture system in space (Spacelab IML-1 Mission).
MedLine Citation:
PMID:  11541489     Owner:  NASA     Status:  MEDLINE    
Cell proliferation, tissue plasminogen activator (t-PA) production and metabolic changes of Hamster Kidney cells (HaK) grown on microcarriers in an automatic Dynamic Cell Culture System (DCCS) were determined on the first International Microgravity Mission (IML-1) Spacelab (22-30 January 1992). The DCCS was designed for two cell culture chambers (volume: 200 microliters each), one operating as a hatch system, the other as a perfusion system. Medium exchange was achieved with an osmotic pump (flow rate 1 microliter h-1). Two major items were investigated: the biological performance of the DCCS in space and the effect of microgravity on HaK cells. The results obtained demonstrated that (1) the DCCS can be used for biological experiments on long term Spacelab missions. In fact, higher cell densities and higher concentration of glucose but lower concentration of lactate in the perfusion chambers than in the batch chambers were measured. The concentration of t-PA, glutamine and ammonia was similar in all chambers. (2) Microgravity had no effect on cell growth and metabolism of HaK cells.
G Lorenzi; F K Gmunder; A Cogoli
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Microgravity science and technology     Volume:  6     ISSN:  0938-0108     ISO Abbreviation:  Microgravity Sci Technol     Publication Date:  1993 Mar 
Date Detail:
Created Date:  1999-03-16     Completed Date:  1999-03-16     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  100971602     Medline TA:  Microgravity Sci Technol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  34-8     Citation Subset:  S    
Space Biology Group, ETH-Technopark, Zurich, Switzerland.
Space Flight Mission:
Flight Experiment; STS-42 Shuttle Project; manned; short duration
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MeSH Terms
Ammonia / metabolism
Cell Culture Techniques / instrumentation*,  methods
Cell Division / physiology
Cells, Cultured
Culture Media
Evaluation Studies as Topic
Glucose / metabolism
Glutamine / metabolism
Hydrogen-Ion Concentration
Kidney / cytology*
Lactic Acid / metabolism
Space Flight / instrumentation*
Tissue Plasminogen Activator / biosynthesis,  metabolism
Reg. No./Substance:
0/Culture Media; 50-21-5/Lactic Acid; 50-99-7/Glucose; 56-85-9/Glutamine; 7664-41-7/Ammonia; 79920-52-8/Cytodex; 9004-54-0/Dextrans; EC Plasminogen Activator

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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