Document Detail

Cultivation of cottontail rabbit epidermal (Sf1Ep) cells on microcarrier beads and their use for suspension cultivation of Treponema pallidum subsp. pallidum.
MedLine Citation:
PMID:  3063209     Owner:  NLM     Status:  MEDLINE    
In vitro propagation of Treponema pallidum can be achieved by cocultivation with Sf1Ep cells. This study had two objectives: (i) to achieve suspension cultivation of Sf1Ep cells and (ii) to develop procedures for achieving the replication of T. pallidum in those cell cultures. Seven suspension cultures of Sf1Ep cells yielded an average of 7.2 x 10(8) T. pallidum (36-fold increase) after 12 days.
B S Riley; D L Cox
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  54     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  1988 Nov 
Date Detail:
Created Date:  1989-02-23     Completed Date:  1989-02-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2862-5     Citation Subset:  IM    
Department of Microbiology and Immunology, Texas College of Osteopathic Medicine, Fort Worth 76107.
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MeSH Terms
Bacteriological Techniques*
Cells, Cultured
Culture Media
Microscopy, Electron, Scanning
Treponema pallidum / growth & development*,  ultrastructure
Grant Support
Reg. No./Substance:
0/Culture Media

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