Document Detail


Cultivation and characterization of novel human group A rotaviruses with long RNA electropherotypes, subgroup II specificities, and serotype 2 VP7 genes.
MedLine Citation:
PMID:  1658036     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
During an epidemiological study of human rotavirus infections in Bangladesh, three group A strains hybridized with a serotype 2 oligonucleotide probe, but they had long RNA electropherotypes. The three strains were collected from 8- to 20-month-old infants with acute diarrhea and moderate malnutrition. By a modified isolation procedure, two strains (T-B and T-C) were adapted in MA104 cell cultures. They were identified to be subgroup II specific by enzyme-linked immunosorbent assay with subgroup I- and II-specific monoclonal antibodies and were identified by a fluorescent focus reduction neutralization assay with hyperimmune antisera to be serotype 2 specific. Further characterization of these unusual rotavirus strains needs to be carried out.
Authors:
F Bingnan; L E Unicomb; G L Tu; A Ali; A Malek; Z Rahim; S Tzipori
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  29     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  1991 Oct 
Date Detail:
Created Date:  1991-12-23     Completed Date:  1991-12-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2224-7     Citation Subset:  IM    
Affiliation:
International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Viral
Antigens, Viral*
Capsid / genetics,  isolation & purification
Capsid Proteins*
Diarrhea / microbiology
Electrophoresis, Polyacrylamide Gel
Genes, Viral
Humans
Infant
RNA, Viral / isolation & purification
Rotavirus / classification*,  genetics,  isolation & purification
Rotavirus Infections / microbiology
Serotyping
Virus Cultivation
Chemical
Reg. No./Substance:
0/Antibodies, Viral; 0/Antigens, Viral; 0/Capsid Proteins; 0/RNA, Viral; 0/VP7 protein, Rotavirus
Comments/Corrections

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