Document Detail


Cucumber root lipoxygenase can act on acyl groups in phosphatidylcholine.
MedLine Citation:
PMID:  9487137     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A cDNA encoding cucumber root lipoxygenase was isolated and expressed in E. coli. The enzyme showed highest activity at pH 5.5 when alpha-linolenic acid dispersed with Tween 20 was used as a substrate but showed little activity at above pH 8.0. On the contrary, it showed the highest activity at pH 9.0 with trilinolenin emulsified with gum arabic. When the assay was performed with linolenic acid dispersed with different concentrations of Tween 20, little activity which could be seen up to the reaction solution became turbid as the linolenic acid/Tween 20 ratio increased, while the activity rapidly emerged afterward. The enzyme could also act on phosphatidylcholine, although the activity was strongly modified by freeze-thaw and sonication treatment on the lipid vesicles. Addition of deoxycholic acid to the phospholipid vesicles drastically enhanced the activity. Addition of free fatty acid was also revealed to be effective to enhance the activity. In the latter case, myristic acid exerted highest activity. Oleic acid enhanced the activity more highly than palmitic acid did. These lines of evidence suggested that the lipoxygenase strictly recognized a specific physical state of the phospholipid substrate in the reaction mixture. The enzyme was irreversibly inactivated as the reaction proceeded, however, the rate of the inactivation was much influenced by the additives. Furthermore, stoichiometry between consumed oxygen and formed conjugated diene could not be observed. (c) 1998 Elsevier Science B.V.
Authors:
K Matsui; M Nishioka; M Ikeyoshi; Y Matsumura; T Mori; T Kajiwara
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1390     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1998 Feb 
Date Detail:
Created Date:  1998-04-24     Completed Date:  1998-04-24     Revised Date:  2001-11-02    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  8-20     Citation Subset:  IM    
Affiliation:
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753, Japan. matsui@agr.yamaguchi-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Cloning, Molecular
Cucumis sativus / enzymology*
DNA, Complementary / chemistry,  isolation & purification
DNA, Plant / chemistry,  isolation & purification
Deoxycholic Acid / pharmacology
Escherichia coli
Fatty Acids / pharmacology
Hydrogen-Ion Concentration
Lipoxygenase / metabolism*
Molecular Sequence Data
Phosphatidylcholines / metabolism*
Plant Roots / enzymology
Surface Properties
Chemical
Reg. No./Substance:
0/DNA, Complementary; 0/DNA, Plant; 0/Fatty Acids; 0/Phosphatidylcholines; 83-44-3/Deoxycholic Acid; EC 1.13.11.12/Lipoxygenase

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