| CuZn-SOD suppresses the bovine papillomavirus-induced proliferation of fibroblasts. | |
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MedLine Citation:
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PMID: 18184412 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Eukaryotic cells continuously produce reactive oxygen species (ROS) and have mechanisms to control ROS levels. ROS have been shown to mediate cell proliferation and transformation. We studied the effect of CuZn-superoxide dismutase (CuZnSOD) on the focus-forming ability of bovine papillomavirus (BPV-1) wtDNA and hypertransforming mutant of its major oncoprotein E5, E5-17S. We found that CuZnSOD suppresses the focus-forming ability of BPV-1 wtDNA and E5 oncoprotein. Significantly fewer foci were detected in pCGCuZnSOD- and BPV-1 DNA-cotransfected cell culture compare to BPV-1 DNA-transfected cell culture (p<0.001). CuZnSOD decreases the rate of cell proliferation in both non-transformed C127 and BPV-1- and E5-transformed cell lines. CuZnSOD decelerates cell entry into the S phase of the cell cycle and has a suppressing effect on the actively dividing cells. As the transformed cells proliferate faster than normal cells when confluent, CuZnSOD inhibits the growth of foci. These results indicate that superoxide radicals may be involved in signaling for cell proliferation and that SOD suppresses cell proliferation. |
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Authors:
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Kai Rausalu; Liis Karo-Astover; Ann Kilk; Mart Ustav |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica Volume: 115 ISSN: 0903-4641 ISO Abbreviation: APMIS Publication Date: 2007 Dec |
Date Detail:
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Created Date: 2008-01-10 Completed Date: 2008-03-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8803400 Medline TA: APMIS Country: Denmark |
Other Details:
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Languages: eng Pagination: 1415-21 Citation Subset: IM |
Affiliation:
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Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bovine papillomavirus 1 / drug effects*, physiology Cattle Cell Proliferation / drug effects* Cell Transformation, Viral / drug effects* Cells, Cultured DNA, Viral Fibroblasts / drug effects, virology* Mice Oncogene Proteins, Viral / drug effects* Papillomavirus Infections / metabolism, virology Superoxide Dismutase / pharmacology* Superoxides / metabolism Transfection |
| Chemical | |
Reg. No./Substance:
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0/DNA, Viral; 0/Oncogene Proteins, Viral; 0/oncogene protein E5, Bovine papillomavirus type 1; 11062-77-4/Superoxides; EC 1.15.1.1/Superoxide Dismutase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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