Document Detail

CuZn-SOD suppresses the bovine papillomavirus-induced proliferation of fibroblasts.
MedLine Citation:
PMID:  18184412     Owner:  NLM     Status:  MEDLINE    
Eukaryotic cells continuously produce reactive oxygen species (ROS) and have mechanisms to control ROS levels. ROS have been shown to mediate cell proliferation and transformation. We studied the effect of CuZn-superoxide dismutase (CuZnSOD) on the focus-forming ability of bovine papillomavirus (BPV-1) wtDNA and hypertransforming mutant of its major oncoprotein E5, E5-17S. We found that CuZnSOD suppresses the focus-forming ability of BPV-1 wtDNA and E5 oncoprotein. Significantly fewer foci were detected in pCGCuZnSOD- and BPV-1 DNA-cotransfected cell culture compare to BPV-1 DNA-transfected cell culture (p<0.001). CuZnSOD decreases the rate of cell proliferation in both non-transformed C127 and BPV-1- and E5-transformed cell lines. CuZnSOD decelerates cell entry into the S phase of the cell cycle and has a suppressing effect on the actively dividing cells. As the transformed cells proliferate faster than normal cells when confluent, CuZnSOD inhibits the growth of foci. These results indicate that superoxide radicals may be involved in signaling for cell proliferation and that SOD suppresses cell proliferation.
Kai Rausalu; Liis Karo-Astover; Ann Kilk; Mart Ustav
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  APMIS : acta pathologica, microbiologica, et immunologica Scandinavica     Volume:  115     ISSN:  0903-4641     ISO Abbreviation:  APMIS     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2008-01-10     Completed Date:  2008-03-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8803400     Medline TA:  APMIS     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  1415-21     Citation Subset:  IM    
Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia.
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MeSH Terms
Bovine papillomavirus 1 / drug effects*,  physiology
Cell Proliferation / drug effects*
Cell Transformation, Viral / drug effects*
Cells, Cultured
DNA, Viral
Fibroblasts / drug effects,  virology*
Oncogene Proteins, Viral / drug effects*
Papillomavirus Infections / metabolism,  virology
Superoxide Dismutase / pharmacology*
Superoxides / metabolism
Reg. No./Substance:
0/DNA, Viral; 0/Oncogene Proteins, Viral; 0/oncogene protein E5, Bovine papillomavirus type 1; 11062-77-4/Superoxides; EC Dismutase

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