Document Detail

CuInS(2) quantum dots as a near-infrared fluorescent probe for detecting thrombin in human serum.
MedLine Citation:
PMID:  23061093     Owner:  NLM     Status:  Publisher    
This paper describes a novel, simple method for the highly sensitive and selective detection of thrombin using fibrinogen (Fib) and CuInS(2) quantum dots (QDs) as biosensing probes. Water-soluble near-infrared CuInS(2) QDs capped by mercaptopropionic acid (MPA) were directly synthesized by a hydrothermal method. Addition of fibrinogen to the CuInS(2) QDs solution led to the formation of a Fib-CuInS(2) QDs complex through electrostatic interactions and hydrogen bonding, and resulting in the enhancement of photoluminescence (PL) intensity and a red shift of the PL peak. Once thrombin was introduced into the Fib-CuInS(2) QDs system, it catalyzed the polymerization of the free and conjugated fibrinogen species to form insoluble fibrillar fibrin-CuInS(2) QDs agglutinates. After centrifugation, the PL intensity of the supernatants decreased upon increasing the concentration of thrombin. This Fib-CuInS(2) QDs probe provided a highly specific selectivity and a linear detection of thrombin in the range of 6.7 × 10(-11) to 3.9 × 10(-7) mol L(-1) with a detection limit (LOD) of about 8.7 × 10(-12) mol L(-1), and realized the thrombin detection in human serum samples directly. Compared with those obtained by using other nanomaterials and aptamer-based detection methods, this approach provided a lower LOD for thrombin detection. The proposed approach provides a simple and fast-responding procedure, which might hold a promising potential for application in the diagnosis of diseases associated with coagulation abnormalities and cancers.
Xue Gao; Xingcen Liu; Zihan Lin; Siyu Liu; Xingguang Su
Related Documents :
24891753 - Surface-bound ruthenium diimine organometallic complexes: excited-state properties.
24402453 - An unprecedented high nuclearity catecholato-based ti(iv)-architecture bearing labile p...
17117213 - Ligand-centred reactivity in diiminepyridine complexes.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-12
Journal Detail:
Title:  The Analyst     Volume:  -     ISSN:  1364-5528     ISO Abbreviation:  Analyst     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372652     Medline TA:  Analyst     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Analytical Chemistry, College of Chemistry, Jilin University, Qianwei Road 10, Changchun, 130012, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Fluorescent polymer-based post-translational differentiation and subtyping of breast cancer cells.
Next Document:  An amine-reactive tetraphenylethylene derivative for protein detection in SDS-PAGE.