| CuInS(2) quantum dots as a near-infrared fluorescent probe for detecting thrombin in human serum. | |
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MedLine Citation:
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PMID: 23061093 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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This paper describes a novel, simple method for the highly sensitive and selective detection of thrombin using fibrinogen (Fib) and CuInS(2) quantum dots (QDs) as biosensing probes. Water-soluble near-infrared CuInS(2) QDs capped by mercaptopropionic acid (MPA) were directly synthesized by a hydrothermal method. Addition of fibrinogen to the CuInS(2) QDs solution led to the formation of a Fib-CuInS(2) QDs complex through electrostatic interactions and hydrogen bonding, and resulting in the enhancement of photoluminescence (PL) intensity and a red shift of the PL peak. Once thrombin was introduced into the Fib-CuInS(2) QDs system, it catalyzed the polymerization of the free and conjugated fibrinogen species to form insoluble fibrillar fibrin-CuInS(2) QDs agglutinates. After centrifugation, the PL intensity of the supernatants decreased upon increasing the concentration of thrombin. This Fib-CuInS(2) QDs probe provided a highly specific selectivity and a linear detection of thrombin in the range of 6.7 × 10(-11) to 3.9 × 10(-7) mol L(-1) with a detection limit (LOD) of about 8.7 × 10(-12) mol L(-1), and realized the thrombin detection in human serum samples directly. Compared with those obtained by using other nanomaterials and aptamer-based detection methods, this approach provided a lower LOD for thrombin detection. The proposed approach provides a simple and fast-responding procedure, which might hold a promising potential for application in the diagnosis of diseases associated with coagulation abnormalities and cancers. |
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Authors:
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Xue Gao; Xingcen Liu; Zihan Lin; Siyu Liu; Xingguang Su |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-10-12 |
Journal Detail:
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Title: The Analyst Volume: - ISSN: 1364-5528 ISO Abbreviation: Analyst Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-10-12 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372652 Medline TA: Analyst Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Analytical Chemistry, College of Chemistry, Jilin University, Qianwei Road 10, Changchun, 130012, China. suxg@jlu.edu.cn. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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