Document Detail


Cu(II) removal by Rhodotorula mucilaginosa RCL-11 in sequential batch cultures.
MedLine Citation:
PMID:  19717909     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present study explored the ability of the yeast Rhodotorula mucilaginosa RCL-11 to adapt to increasing Cu(II) concentrations, measuring oxidative stress through superoxide dismutase and catalase activity in two parallel sequential batch assays. One assay was performed in Erlenmeyer flasks without aeration and a second in a fermentor in which the dissolved oxygen was maintained at 30% saturation. Both assays were carried out by increasing Cu(II) concentrations in five sequential steps: 0; 0.1; 0.2; 0.5 and 1 mM. Each assay was incubated at 30 degrees C, 250 rpm and pH 5.5. While growth parameters of R. mucilaginosa RCL-11 decreased 90-95% with increasing Cu(II) concentration in the culture medium, the oxidative stress level increased from 30 to 55% in both assays. Cells grown under controlled oxygen conditions showed 30% more copper bioaccumulation and 10% glucose consumption when compared with cells grown without aeration. SOD activity was higher under controlled than without aeration, whereas CAT activity was similar under both test conditions. Cu(II) bioaccumulation by R. mucilaginosa RCL-11 and a possible increase in this capacity by adaptation of the strain under controlled aeration represent a potential valuable tool for treatment of effluents or water bioremediation with high copper contents.
Authors:
Liliana B Villegas; María J Amoroso; Lucía I C de Figueroa; Faustino Siñeriz; Faustino Siñeriz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Water science and technology : a journal of the International Association on Water Pollution Research     Volume:  60     ISSN:  0273-1223     ISO Abbreviation:  Water Sci. Technol.     Publication Date:  2009  
Date Detail:
Created Date:  2009-08-31     Completed Date:  2009-10-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9879497     Medline TA:  Water Sci Technol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1225-32     Citation Subset:  IM    
Affiliation:
PROIMI-CONICET, Av. Belgrano y Pje. Caseros. T4001MVB Tucumán, Argentina. lbvilleg@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Biodegradation, Environmental
Bioreactors*
Catalase / metabolism
Copper / isolation & purification*,  metabolism*,  toxicity
Environmental Pollutants / isolation & purification*,  metabolism*,  toxicity
Fermentation
Oxidative Stress / drug effects
Rhodotorula / drug effects,  enzymology,  growth & development*,  metabolism*
Superoxide Dismutase / metabolism
Chemical
Reg. No./Substance:
0/Environmental Pollutants; 7440-50-8/Copper; EC 1.11.1.6/Catalase; EC 1.15.1.1/Superoxide Dismutase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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