| Crystallization and some properties of glutamate dehydrogenase from rat liver. | |
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MedLine Citation:
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PMID: 11452377 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. Glutamate dehydrogenase (L-glutamate:NAD(P) oxidoreductase, EC 1.4.1.3) from rat liver has been crystallized with a method carefully avoiding all denaturating agents. A 236-fold purification was achieved at a yield of 20%. The specific activity was 185 units/mg protein. The enzyme was homogeneous by analytical zone electrophoresis and sedimentation studies. The s0(20),w value was 13.2. 2. Sedimentation studies in the analytical ultracentrifuge and the behaviour of the enzyme in the disc-electrophoresis revealed that glutamate dehydrogenase from rat liver did not undergo a reversible association-dissociation reaction as reported of glutamate dehydrogenase of nearly all other mammalians. 3. Using antibodies prepared against crystalline bovine liver glutamate dehydrogenase, no immunological differences between the rat and the bovine liver enzyme could be observed. |
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Authors:
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H Arnold; K P Maier |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 251 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1971 Nov |
Date Detail:
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Created Date: 2001-07-16 Completed Date: 2001-08-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 133-40 Citation Subset: IM |
Affiliation:
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Universität Konstanz und Medizinische Universitätsklinik Freiburg i.Br., Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle Crystallization Glutamate Dehydrogenase / chemistry*, isolation & purification, metabolism* Kinetics Liver / enzymology* Mitochondria, Liver / enzymology Rats |
| Chemical | |
Reg. No./Substance:
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EC 1.4.1.2/Glutamate Dehydrogenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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