| Crystallization and preliminary crystallographic analysis of a chitinase from Clonostachys rosea. | |
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MedLine Citation:
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PMID: 19342787 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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CrChi1 is a chitinase from the nematophagous fungus Clonostachys rosea that plays a role in the infection of nematodes. In order to resolve the crystal structure of CrChi1 and to gain a better understanding of its biological functions, recombinant CrChi1 was crystallized at 291 K using PEG 3350 and ammonium dihydrogen phosphate as precipitant and a 1.8 A resolution X-ray data set was collected from a single flash-cooled crystal (100 K). The crystals belonged to space group P2(1), with unit-cell parameters a = 44.1, b = 71.7, c = 59.1 A, alpha = gamma = 90, beta = 91.3 degrees. Assuming the presence of one molecule per asymmetric unit, the Matthews coefficient and solvent content were calculated to be 2.45 A(3) Da(-1) and 40%, respectively. To our knowledge, this is the first structure determination study of a chitinase from a nematophagous fungus. |
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Authors:
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Zhongwei Gan; Jinkui Yang; Nan Tao; Zhiyong Lou; Qili Mi; Zhaohui Meng; Ke Qin Zhang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-03-25 |
Journal Detail:
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Title: Acta crystallographica. Section F, Structural biology and crystallization communications Volume: 65 ISSN: 1744-3091 ISO Abbreviation: Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. Publication Date: 2009 Apr |
Date Detail:
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Created Date: 2009-04-03 Completed Date: 2009-05-01 Revised Date: 2013-04-11 |
Medline Journal Info:
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Nlm Unique ID: 101226117 Medline TA: Acta Crystallogr Sect F Struct Biol Cryst Commun Country: England |
Other Details:
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Languages: eng Pagination: 386-8 Citation Subset: IM |
Affiliation:
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Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, Kunming 650091, People's Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Chitinase
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chemistry* Crystallization Crystallography, X-Ray Hypocreales / enzymology* |
| Chemical | |
Reg. No./Substance:
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EC 3.2.1.14/Chitinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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