| Crystallization of and preliminary X-ray diffraction data for TET-repressor and the TET-repressor-tetracycline complex. | |
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MedLine Citation:
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PMID: 6527388 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The TET-repressor encoded by the transposon Tn10 has been crystallized along with the repressor-tetracycline complex. Both crystals belong to the space group P43212 (or P41212) with cell dimensions a = b = 74.3(1) A, c = 94.2(2) A and a = b = 73.3(1) A, c = 94.6(2) A for the free and complexed repressor, respectively. There is one molecule of molecular weight 23,000 per asymmetric unit, and the biologically active dimer therefore consists of two identically formed subunits which are related by a crystallographic 2-fold axis. This isomorphism of TET-repressor and its tetracycline complex suggests that only minor, subtle changes in structure trigger binding to or release of the operator. The crystals of the native protein permit X-ray data collection to 3.2 A and those of the complexed repressor to 2.8 A. |
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Authors:
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H E Parge; M Schneider; V Hahn; W Saenger; L Altschmied; W Hillen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of molecular biology Volume: 180 ISSN: 0022-2836 ISO Abbreviation: J. Mol. Biol. Publication Date: 1984 Dec |
Date Detail:
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Created Date: 1985-04-18 Completed Date: 1985-04-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985088R Medline TA: J Mol Biol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1189-91 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Crystallization Macromolecular Substances Repressor Proteins* Tetracycline* Transcription Factors* X-Ray Diffraction |
| Chemical | |
Reg. No./Substance:
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0/Macromolecular Substances; 0/Repressor Proteins; 0/Transcription Factors; 0/tetracycline resistance-encoding transposon repressor protein; 60-54-8/Tetracycline |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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