Document Detail


Crystallization and preliminary X-ray analysis of ferric enterobactin receptor FepA, an integral membrane protein from Escherichia coli.
MedLine Citation:
PMID:  9761879     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Diffraction-quality crystals have been obtained of the integral membrane protein ferric enterobactin receptor (FepA) from the outer membrane of Escherichia coli. Crystals were grown using the zwitterionic detergent lauryldimethylamine oxide (LDAO), the precipitants polyethylene glycol (PEG) 1000 and sodium chloride, and the additive heptane-1,2,3-triol; they have the symmetry of the orthorhomic space group C2221 with a = 112.2, b = 137.2 and c = 135. 4 A and diffract to 2.5 A resolution. The crystals were flash-cooled and a preliminary data set was collected at 103 K. The crystals are suitable for three-dimensional structure analysis.
Authors:
B S Smith; B Kobe; R Kurumbail; S K Buchanan; L Venkatramani; D Van Der Helm; J Deisenhofer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Acta crystallographica. Section D, Biological crystallography     Volume:  54     ISSN:  0907-4449     ISO Abbreviation:  Acta Crystallogr. D Biol. Crystallogr.     Publication Date:  1998 Jul 
Date Detail:
Created Date:  1998-12-14     Completed Date:  1998-12-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9305878     Medline TA:  Acta Crystallogr D Biol Crystallogr     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  697-9     Citation Subset:  IM    
Affiliation:
Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, Texas 75235-9050, USA.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Outer Membrane Proteins / chemistry*,  isolation & purification
Carrier Proteins / chemistry*,  isolation & purification
Crystallization
Crystallography, X-Ray
Escherichia coli / enzymology*
Protein Conformation
Receptors, Cell Surface / chemistry*,  isolation & purification
Grant Support
ID/Acronym/Agency:
GM21822/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Outer Membrane Proteins; 0/Carrier Proteins; 0/Receptors, Cell Surface; 0/enterobactin receptor

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