| Crystallization and preliminary X-ray analysis of ferric enterobactin receptor FepA, an integral membrane protein from Escherichia coli. | |
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MedLine Citation:
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PMID: 9761879 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Diffraction-quality crystals have been obtained of the integral membrane protein ferric enterobactin receptor (FepA) from the outer membrane of Escherichia coli. Crystals were grown using the zwitterionic detergent lauryldimethylamine oxide (LDAO), the precipitants polyethylene glycol (PEG) 1000 and sodium chloride, and the additive heptane-1,2,3-triol; they have the symmetry of the orthorhomic space group C2221 with a = 112.2, b = 137.2 and c = 135. 4 A and diffract to 2.5 A resolution. The crystals were flash-cooled and a preliminary data set was collected at 103 K. The crystals are suitable for three-dimensional structure analysis. |
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Authors:
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B S Smith; B Kobe; R Kurumbail; S K Buchanan; L Venkatramani; D Van Der Helm; J Deisenhofer |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Acta crystallographica. Section D, Biological crystallography Volume: 54 ISSN: 0907-4449 ISO Abbreviation: Acta Crystallogr. D Biol. Crystallogr. Publication Date: 1998 Jul |
Date Detail:
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Created Date: 1998-12-14 Completed Date: 1998-12-14 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9305878 Medline TA: Acta Crystallogr D Biol Crystallogr Country: DENMARK |
Other Details:
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Languages: eng Pagination: 697-9 Citation Subset: IM |
Affiliation:
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Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, Texas 75235-9050, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Outer Membrane Proteins
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chemistry*,
isolation & purification Carrier Proteins / chemistry*, isolation & purification Crystallization Crystallography, X-Ray Escherichia coli / enzymology* Protein Conformation Receptors, Cell Surface / chemistry*, isolation & purification |
| Grant Support | |
ID/Acronym/Agency:
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GM21822/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Outer Membrane Proteins; 0/Carrier Proteins; 0/Receptors, Cell Surface; 0/enterobactin receptor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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