Document Detail


Crystallization and preliminary X-ray analysis of the primary receptor (PotD) of the polyamine transport system in Escherichia coli.
MedLine Citation:
PMID:  15299717     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
The primary receptor (potD, M(r) = 39 000) of the polyamine transport system in Escherichia coli has been crystallized by the vapor-diffusion method. Two crystal forms were obtained in the presence of spermidine, and were examined by X-ray analysis. Form I crystals, which diffract to 2.5 A resolution, belong to the space group P2(1), with unit-cell dimensions a = 145.3, b = 69.1, c = 72.5 A and beta = 107.6 degrees. Four molecules are contained in an asymmetric unit. These form two dimers that are related to each other by a local translation of about half of the unit cell along the a axis. The two protein molecules in each dimer are similarly related by a local dyad. Form II crystals diffract to 1.8 A resolution and belong to the space group I4(1), with unit-cell dimensions a = b = 130.3 and c = 38.7 A. They contain one molecule per asymmetric unit.
Authors:
S Sugiyama; M Matsushima; T Saisho; K Kashiwagi; K Igarashi; K Morikawa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta crystallographica. Section D, Biological crystallography     Volume:  52     ISSN:  0907-4449     ISO Abbreviation:  Acta Crystallogr. D Biol. Crystallogr.     Publication Date:  1996 Mar 
Date Detail:
Created Date:  2004-08-09     Completed Date:  2005-02-15     Revised Date:  2007-07-24    
Medline Journal Info:
Nlm Unique ID:  9305878     Medline TA:  Acta Crystallogr D Biol Crystallogr     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  416-8     Citation Subset:  -    
Affiliation:
Protein Engineering Research Institute, Suita, Osaka, Japan.
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