| Crystallization and X-ray diffraction analysis of an 'all-locked' nucleic acid duplex derived from a tRNA(Ser) microhelix. | |
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MedLine Citation:
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PMID: 19652346 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Modified nucleic acids are of great interest with respect to their nuclease resistance and enhanced thermostability. In therapeutical and diagnostic applications, such molecules can substitute for labile natural nucleic acids that are targeted against particular diseases or applied in gene therapy. The so-called 'locked nucleic acids' contain modified sugar moieties such as 2'-O,4'-C-methylene-bridged beta-D-ribofuranose and are known to be very stable nucleic acid derivatives. The structure of locked nucleic acids in single or multiple LNA-substituted natural nucleic acids and in LNA-DNA or LNA-RNA heteroduplexes has been well investigated, but the X-ray structure of an ;all-locked' nucleic acid double helix has not been described to date. Here, the crystallization and X-ray diffraction data analysis of an 'all-locked' nucleic acid helix, which was designed as an LNA originating from a tRNA(Ser) microhelix RNA structure, is presented. The crystals belonged to space group C2, with unit-cell parameters a = 77.91, b = 40.74, c = 30.06 A, beta = 91.02 degrees . A high-resolution and a low-resolution data set were recorded, with the high-resolution data showing diffraction to 1.9 A resolution. The crystals contained two double helices per asymmetric unit, with a Matthews coefficient of 2.48 A(3) Da(-1) and a solvent content of 66.49% for the merged data. |
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Authors:
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Katja Behling; André Eichert; Jens P Fürste; Christian Betzel; Volker A Erdmann; Charlotte Förster |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-07-30 |
Journal Detail:
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Title: Acta crystallographica. Section F, Structural biology and crystallization communications Volume: 65 ISSN: 1744-3091 ISO Abbreviation: Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-08-04 Completed Date: 2009-12-11 Revised Date: 2011-08-03 |
Medline Journal Info:
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Nlm Unique ID: 101226117 Medline TA: Acta Crystallogr Sect F Struct Biol Cryst Commun Country: England |
Other Details:
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Languages: eng Pagination: 809-12 Citation Subset: IM |
Affiliation:
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Institute of Chemistry and Biochemistry, Free University Berlin, 14195 Berlin, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Crystallization Crystallography, X-Ray Models, Molecular Nucleic Acid Conformation* Nucleic Acids / chemistry* RNA, Transfer, Ser / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Nucleic Acids; 0/RNA, Transfer, Ser |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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