Document Detail

Crystalline silica induces apoptosis in human endothelial cells in vitro.
MedLine Citation:
PMID:  15242185     Owner:  NLM     Status:  MEDLINE    
We investigated whether incubation of cultured human aortic endothelial cells (HAEC) with crystalline silica at the concentration 1 cm2/ml (chosen on the basis of a pilot experiment) leads to alterations typical of apoptosis. The binding of annexin V as early, and DNA fragmentation as late events of apoptosis were measured besides the number of cells with depolarized mitochondria. The generation of reactive oxygen species (ROS) by HAEC in presence of silica was determined as well as silica ability to in vitro generate hydroxyl radicals was investigated. After 18 h of silica incubation, about 30% of viable cells bound annexin V. After 24 h of silica treatment, the percentage of cells with fragmented DNA (Tunel positive) was 27% and it increased up to 50% after 48 h, whereas in untreated cells this percentage was 7% and 11% after 24 and 48 h, respectively. The presence of fragmented DNA in cells treated with silica was confirmed by agarose gel electrophoresis. In agreement with these results showing an induction of HAEC apoptosis by silica incubation, the number of cells with depolarized mitochondria was significantly higher after silica treatment as compared to the control. Apoptosis was also obtained with silica added to aliquots of anti-C5a-absorbed-medium. In the cells exposed to silica there was a significant increasing of ROS generation in comparison to the untreated cells. Apoptosis might be due to peroxidative stress since silica can generate hydroxyl radicals.
L Santarelli; R Recchioni; F Moroni; F Marcheselli; M Governa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biology and toxicology     Volume:  20     ISSN:  0742-2091     ISO Abbreviation:  Cell Biol. Toxicol.     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2004-07-09     Completed Date:  2004-12-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8506639     Medline TA:  Cell Biol Toxicol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  97-108     Citation Subset:  IM    
Department of Molecular Pathology-Occupational Medicine Branch, Polytechnical University of Marche, Ancona, Italy.
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MeSH Terms
Annexin A5 / metabolism
Apoptosis / drug effects*
Cells, Cultured
DNA Fragmentation
Electrophoresis, Agar Gel
Endothelium, Vascular / drug effects*,  metabolism,  pathology
Hydroxyl Radical / metabolism
In Situ Nick-End Labeling
Membrane Potentials / drug effects
Mitochondria / drug effects
Quartz / toxicity*
Reactive Oxygen Species / metabolism
Reg. No./Substance:
0/Annexin A5; 0/Reactive Oxygen Species; 14808-60-7/Quartz; 3352-57-6/Hydroxyl Radical

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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