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CRYSTALLINE RIBONUCLEASE.
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MedLine Citation:
PMID:  19873197     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
1. A crystalline enzyme capable of digesting yeast nucleic acid has been isolated from fresh beef pancreas. 2. The enzyme called "ribonuclease" is a soluble protein of albumin type. Its molecular weight is about 15,000. Its isoelectric point is in the region of pH 8.0. 3. Ribonuclease splits yeast nucleic acid into fragments small enough to diffuse readily through collodion or cellophane membranes. 4. The split products of digestion, unlike the undigested yeast nucleic acid, are not precipitable with glacial acetic acid or dilute hydrochloric acid. 5. The digestion of yeast nucleic acid is accompanied by a gradual formation of free acid groups without any significant liberation of free phosphoric acid. 6. Ribonuclease is stable over a wide range of pH even when heated for a short time at 100 degrees C. Its maximum stability is in the range of pH 2.0 to 4.5. 7. Denaturation of the protein of ribonuclease by heat or alkali, or digestion of the protein by pepsin, causes a corresponding percentage loss in the enzymatic activity of the material.
Authors:
M Kunitz
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of general physiology     Volume:  24     ISSN:  0022-1295     ISO Abbreviation:  J. Gen. Physiol.     Publication Date:  1940 Sep 
Date Detail:
Created Date:  2010-06-22     Completed Date:  2010-06-22     Revised Date:  2010-09-28    
Medline Journal Info:
Nlm Unique ID:  2985110R     Medline TA:  J Gen Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  15-32     Citation Subset:  -    
Affiliation:
Laboratories of The Rockefeller Institute for Medical Research, Princeton, New Jersey.
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Full Text
Journal Information
Journal ID (nlm-ta): J Gen Physiol
ISSN: 0022-1295
ISSN: 1540-7748
Publisher: The Rockefeller University Press
Article Information
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Copyright © Copyright, 1940, by The Rockefeller Institute for Medical Research
Received Day: 3 Month: 6 Year: 1940
Print publication date: Day: 20 Month: 9 Year: 1940
Volume: 24 Issue: 1
First Page: 15 Last Page: 32
ID: 2142070
PubMed Id: 19873197

CRYSTALLINE RIBONUCLEASE
M. Kunitz
From the Laboratories of The Rockefeller Institute for Medical Research, Princeton, New Jersey


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