| CRYSTALLINE RIBONUCLEASE. | |
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MedLine Citation:
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PMID: 19873197 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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1. A crystalline enzyme capable of digesting yeast nucleic acid has been isolated from fresh beef pancreas. 2. The enzyme called "ribonuclease" is a soluble protein of albumin type. Its molecular weight is about 15,000. Its isoelectric point is in the region of pH 8.0. 3. Ribonuclease splits yeast nucleic acid into fragments small enough to diffuse readily through collodion or cellophane membranes. 4. The split products of digestion, unlike the undigested yeast nucleic acid, are not precipitable with glacial acetic acid or dilute hydrochloric acid. 5. The digestion of yeast nucleic acid is accompanied by a gradual formation of free acid groups without any significant liberation of free phosphoric acid. 6. Ribonuclease is stable over a wide range of pH even when heated for a short time at 100 degrees C. Its maximum stability is in the range of pH 2.0 to 4.5. 7. Denaturation of the protein of ribonuclease by heat or alkali, or digestion of the protein by pepsin, causes a corresponding percentage loss in the enzymatic activity of the material. |
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Authors:
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M Kunitz |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of general physiology Volume: 24 ISSN: 0022-1295 ISO Abbreviation: J. Gen. Physiol. Publication Date: 1940 Sep |
Date Detail:
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Created Date: 2010-06-22 Completed Date: 2010-06-22 Revised Date: 2010-09-28 |
Medline Journal Info:
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Nlm Unique ID: 2985110R Medline TA: J Gen Physiol Country: United States |
Other Details:
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Languages: eng Pagination: 15-32 Citation Subset: - |
Affiliation:
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Laboratories of The Rockefeller Institute for Medical Research, Princeton, New Jersey. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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Journal Information Journal ID (nlm-ta): J Gen Physiol ISSN: 0022-1295 ISSN: 1540-7748 Publisher: The Rockefeller University Press |
Article Information Download PDF  Copyright © Copyright, 1940, by The Rockefeller Institute for Medical Research Received Day: 3 Month: 6 Year: 1940 Print publication date: Day: 20 Month: 9 Year: 1940 Volume: 24 Issue: 1 First Page: 15 Last Page: 32 ID: 2142070 PubMed Id: 19873197 |
| CRYSTALLINE RIBONUCLEASE | |
| M. Kunitz | |
| From the Laboratories of The Rockefeller Institute for Medical Research, Princeton, New Jersey |
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