Document Detail

Crystal structures of two mutants of adenylate kinase from Escherichia coli that modify the Gly-loop.
MedLine Citation:
PMID:  8451239     Owner:  NLM     Status:  MEDLINE    
Two mutants of adenylate kinase from Escherichia coli have been crystallized and analyzed by X-ray diffraction at resolutions of 3.4 and 2.4 A, respectively. These mutants are Pro-9-->Leu and Gly-10-->Val. They were selected for their positions in the highly conserved Gly-loop forming a giant anion hole for the beta-phosphate of ATP (GTP) in adenylate kinases, H-ras-p21, and other nucleotide-binding proteins. Mutants at these positions of H-ras-p21 cause cancer. In adenylate kinase these mutations cause smallish changes at the active site. Relating the structural changes to the known changes in catalysis indicates that these mutants hinder the induced-fit movements. As a side result we find that mutant Pro-9-->Leu and wild-type form one very similar crystal packing contact that is crystallographic in one case and noncrystallographic in the other, while all other packing contacts and the space groups are quite at variance.
C W Müller; G E Schulz
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Proteins     Volume:  15     ISSN:  0887-3585     ISO Abbreviation:  Proteins     Publication Date:  1993 Jan 
Date Detail:
Created Date:  1993-04-14     Completed Date:  1993-04-14     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8700181     Medline TA:  Proteins     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  42-9     Citation Subset:  IM    
Institut für Organische Chemie und Biochemie der Universität, Freiburg im Breisgau, Federal Republic of Germany.
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MeSH Terms
Adenylate Kinase / chemistry*,  isolation & purification
Carrier Proteins / chemistry
Cyclic AMP Receptor Protein*
Escherichia coli / enzymology*
Models, Chemical
Models, Molecular
Protein Conformation
Proto-Oncogene Proteins p21(ras) / chemistry
Receptors, Cyclic AMP / chemistry
X-Ray Diffraction
Reg. No./Substance:
0/Carrier Proteins; 0/Cyclic AMP Receptor Protein; 0/Receptors, Cyclic AMP; EC Kinase; EC Proteins p21(ras)

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