Document Detail


Crystal structure of the transcriptional activator HlyU from Vibrio vulnificus CMCP6.
MedLine Citation:
PMID:  20178784     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
HlyU is a transcription factor of the ArsR/SmtB family and activates the expression of the pathogenic Vibrio vulnificus RTX toxin. In contrast to the other metal-responding ArsR/SmtB proteins, HlyU does not sense metal ions. To provide its structural information, we elucidated the crystal structure of HlyU from V. vulnificus CMCP6 (HlyU_Vv). The monomeric HlyU_Vv architecture of five alpha-helices and two beta-strands, some of which constitute a typical DNA-binding winged helix-turn-helix (wHTH) motif, is very similar to that of other transcription regulators. Nonetheless, the homo-dimeric HlyU_Vv structure shows several different, three-dimensional features in the spatial position and the detailed dimeric interaction, which were not observed in the modeling study based on the same protein family and sequence similarity.
Authors:
Kosuke Nishi; Hyun-Ju Lee; Suk-Youl Park; Soo Jang Bae; Shee Eun Lee; Paul D Adams; Joon Haeng Rhee; Jeong-Sun Kim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-02-21
Journal Detail:
Title:  FEBS letters     Volume:  584     ISSN:  1873-3468     ISO Abbreviation:  FEBS Lett.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-03-15     Completed Date:  2010-04-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1097-102     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Federation of European Biochemical Societies. All rights reserved.
Affiliation:
Department of Chemistry, Chonnam National University, Gwangju, Republic of Korea.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Binding Sites
Crystallography, X-Ray
DNA, Bacterial / metabolism
Hemolysin Factors / chemistry*,  metabolism
Metals, Heavy / metabolism
Models, Molecular
Molecular Sequence Data
Protein Binding
Protein Conformation
Protein Multimerization
Sequence Homology, Amino Acid
Trans-Activators / chemistry,  metabolism
Vibrio vulnificus* / genetics,  metabolism
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/Metals, Heavy; 0/Trans-Activators

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