| Crystal structure of ribosomal protein L4 shows RNA-binding sites for ribosome incorporation and feedback control of the S10 operon. | |
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MedLine Citation:
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PMID: 10698923 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ribosomal protein L4 resides near the peptidyl transferase center of the bacterial ribosome and may, together with rRNA and proteins L2 and L3, actively participate in the catalysis of peptide bond formation. Escherichia coli L4 is also an autogenous feedback regulator of transcription and translation of the 11 gene S10 operon. The crystal structure of L4 from Thermotoga maritima at 1.7 A resolution shows the protein with an alternating alpha/beta fold and a large disordered loop region. Two separate binding sites for RNA are discernible. The N-terminal site, responsible for binding to rRNA, consists of the disordered loop with flanking alpha-helices. The C-terminal site, a prime candidate for the interaction with the leader sequence of the S10 mRNA, involves two non-consecutive alpha-helices. The structure also suggests a C-terminal protein-binding interface, through which L4 could be interacting with protein components of the transcriptional and/or translational machineries. |
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Authors:
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M Worbs; R Huber; M C Wahl |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The EMBO journal Volume: 19 ISSN: 0261-4189 ISO Abbreviation: EMBO J. Publication Date: 2000 Mar |
Date Detail:
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Created Date: 2000-04-26 Completed Date: 2000-04-26 Revised Date: 2013-04-18 |
Medline Journal Info:
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Nlm Unique ID: 8208664 Medline TA: EMBO J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 807-18 Citation Subset: IM |
Affiliation:
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Max-Planck-Institut für Biochemie, Abteilung Strukturforschung, Am Klopferspitz 18a, D-82152 Martinsried, Germany. worbs@biochem.mpg.de |
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Binding Sites Escherichia coli Molecular Sequence Data Nucleic Acid Conformation Operon Protein Binding Protein Conformation RNA, Ribosomal / metabolism Ribosomal Proteins / chemistry*, genetics, metabolism Structure-Activity Relationship Thermotoga maritima |
| Chemical | |
Reg. No./Substance:
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0/RNA, Ribosomal; 0/Ribosomal Proteins; 0/ribosomal protein L4 |
| Comments/Corrections | |
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