| Crystal structure of porcine ribonuclease inhibitor, a protein with leucine-rich repeats. | |
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MedLine Citation:
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PMID: 8264799 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ribonuclease inhibitor is a cytoplasmic protein that tightly binds and inhibits ribonucleases of the pancreatic ribonuclease superfamily. The primary sequence of this inhibitor contains leucine-rich repeats (LRRs); these motifs are present in many proteins that participate in protein-protein interactions and have different functions and cellular locations. In vivo, ribonuclease inhibitor may have a role in the regulation of RNA turnover in mammalian cells and in angiogenesis. To define the structural features of LRR proteins and to understand better the nature of the tight interaction of ribonuclease inhibitor with ribonucleases, we have determined the crystal structure of the porcine inhibitor. To our knowledge, this is the first three-dimensional structure of a protein containing LRRs and represents a new class of alpha/beta protein fold. Individual repeats constitute beta-alpha structural units that probably also occur in other proteins containing LRRs. The non-globular shape of the structure and the exposed face of the parallel beta-sheet may explain why LRRs are used to achieve strong protein-protein interactions. A possible ribonuclease-binding region incorporates the surface formed by the parallel beta-sheet and the beta alpha loops. |
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Authors:
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B Kobe; J Deisenhofer |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Nature Volume: 366 ISSN: 0028-0836 ISO Abbreviation: Nature Publication Date: 1993 Dec 23-30 |
Date Detail:
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Created Date: 1994-01-21 Completed Date: 1994-01-21 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0410462 Medline TA: Nature Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 751-6 Citation Subset: IM |
Affiliation:
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Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas 75235-9050. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Computer Graphics Crystallography Leucine / chemistry* Models, Molecular Molecular Sequence Data Protein Conformation Proteins / chemistry* Ribonucleases / antagonists & inhibitors* Swine |
| Chemical | |
Reg. No./Substance:
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0/Proteins; 39369-21-6/ribonuclease inhibitor, porcine; 61-90-5/Leucine; EC 3.1.-/Ribonucleases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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