Document Detail


The crystal structure of α-Dioxygenase provides insight into diversity in the cyclooxygenase-peroxidase superfamily.
MedLine Citation:
PMID:  23373518     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
α-Dioxygenases (α-DOX) oxygenate fatty acids into 2(R)-hydroperoxides. Despite the low level of sequence identity, α-DOX share common catalytic features with cyclooxygenases (COX), including the use of a tyrosyl radical during catalysis. We determined the X-ray crystal structure of Arabidopsis thaliana α-DOX to 1.5 Å resolution. The α-DOX structure is monomeric, predominantly α-helical, and comprised of two domains. The base domain exhibits a low degree of structural homology with the membrane-binding domain of COX but lies in a similar position with respect to the catalytic domain. The catalytic domain shows the highest degree of similarity with the COX catalytic domain, where 21 of the 22 α-helical elements are conserved. Helices H2, H6, H8, and H17 form the heme binding cleft and walls of the active site channel. His-318, Thr-323, and Arg-566 are located near the catalytic tyrosine, Tyr-386, at the apex of the channel, where they interact with a chloride ion. Substitutions at these positions coupled with kinetic analyses confirm previous hypotheses that implicate these residues as being involved in binding and orienting the carboxylate group of the fatty acid for optimal catalysis. Unique to α-DOX is the presence of two extended inserts on the surface of the enzyme that restrict access to the distal face of the heme, providing an explanation for the observed reduced peroxidase activity of the enzyme. The α-DOX structure represents the first member of the α-DOX subfamily to be structurally characterized within the cyclooxygenase-peroxidase family of heme-containing proteins.
Authors:
Christopher C Goulah; Guangyu Zhu; Mary Koszelak-Rosenblum; Michael G Malkowski
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2013-02-14
Journal Detail:
Title:  Biochemistry     Volume:  52     ISSN:  1520-4995     ISO Abbreviation:  Biochemistry     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-02-27     Completed Date:  2013-04-15     Revised Date:  2014-03-19    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1364-72     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Arabidopsis / chemistry,  enzymology*,  metabolism
Binding Sites
Calcium / metabolism
Catalytic Domain
Crystallography, X-Ray
Dioxygenases / chemistry*,  metabolism
Heme / metabolism
Mice
Models, Molecular
Peroxidase / chemistry,  metabolism
Prostaglandin-Endoperoxide Synthases / chemistry,  metabolism
Protein Structure, Secondary
Protein Structure, Tertiary
Grant Support
ID/Acronym/Agency:
P41 GM103485/GM/NIGMS NIH HHS; R01 GM077176/GM/NIGMS NIH HHS; R01GM077176/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
42VZT0U6YR/Heme; EC 1.11.1.7/Peroxidase; EC 1.13.11.-/Dioxygenases; EC 1.14.99.1/Prostaglandin-Endoperoxide Synthases; SY7Q814VUP/Calcium
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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