Document Detail

Crystal structure of the complex of concanavalin A and tripeptide.
MedLine Citation:
PMID:  11330349     Owner:  NLM     Status:  MEDLINE    
The X-ray structure analysis of a cross-linked crystal of concanavalin A soaked with the tripeptide molecule as the probe molecule showed electron density corresponding to full occupation in the binding pocket. The site lies on the surface of concanavalin A and is surrounded by three symmetry-related molecules. The crystal structure of the tripeptide complex was refined at 2.4-A resolution to an R-factor of 17.5%, (Rfree factor of 23.7%), with an RMS deviation in bond distances of 0.01 A. The model includes all 237 residue of concanavalin A, 1 manganese ion, 1 calcium ion, 161 water molecules, 1 glutaraldehyde molecule, and 1 tripeptide molecule. This X-ray structure analysis also provides an approach to mapping the binding surface of crystalline protein with a probe molecule that is dissolved in a mixture of organic solvent with water or in neat organic solvent but is hardly dissolved in aqueous solution.
Z Zhang; M Qian; Q Huang; Y Jia; Y Tang; K Wang; D Cui; M Li
Related Documents :
8027999 - Tertiary structure of erabutoxin b in aqueous solution as elucidated by two-dimensional...
22136329 - Synthesis, structure, and magnetism of an f element nitrosyl complex, (c5me4h)3uno.
22951589 - Charge effect of foreign metal ions and the crystal growth process in hybridized metal-...
25356529 - An nmr method for the quantitative assessment of intramolecular hydrogen bonding; appli...
22098459 - Structural, magnetic, and electronic properties of phenolic oxime complexes of cu and ni.
21655629 - Insights on the photomagnetism in copper octacyanomolybdates.
22012549 - Sulfonate-tagged 1,4-diazabutadiene (dad(s)) ligands and their noble-metal complexes - ...
21566189 - Beating crystallization in glass-forming metals by millisecond heating and processing.
24588769 - Chiral phase transfer and enantioenrichment of thiolate-protected au102 clusters.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of protein chemistry     Volume:  20     ISSN:  0277-8033     ISO Abbreviation:  J. Protein Chem.     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2001-05-01     Completed Date:  2001-10-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8217321     Medline TA:  J Protein Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  59-65     Citation Subset:  IM    
Department of Chemistry, Peking University, Beijing, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Binding Sites
Concanavalin A / chemistry*,  metabolism*
Crystallography, X-Ray
Models, Molecular
Peptides / chemistry,  metabolism*
Protein Conformation
Reg. No./Substance:
0/Peptides; 11028-71-0/Concanavalin A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Kinetic and structural properties of two isoforms of trypsin isolated from the viscera of Japanese a...
Next Document:  Reactivation kinetics of guanidine hydrochloride-denatured creatine kinase measured using the substr...