Document Detail


Crystal structure of the complex of concanavalin A and tripeptide.
MedLine Citation:
PMID:  11330349     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The X-ray structure analysis of a cross-linked crystal of concanavalin A soaked with the tripeptide molecule as the probe molecule showed electron density corresponding to full occupation in the binding pocket. The site lies on the surface of concanavalin A and is surrounded by three symmetry-related molecules. The crystal structure of the tripeptide complex was refined at 2.4-A resolution to an R-factor of 17.5%, (Rfree factor of 23.7%), with an RMS deviation in bond distances of 0.01 A. The model includes all 237 residue of concanavalin A, 1 manganese ion, 1 calcium ion, 161 water molecules, 1 glutaraldehyde molecule, and 1 tripeptide molecule. This X-ray structure analysis also provides an approach to mapping the binding surface of crystalline protein with a probe molecule that is dissolved in a mixture of organic solvent with water or in neat organic solvent but is hardly dissolved in aqueous solution.
Authors:
Z Zhang; M Qian; Q Huang; Y Jia; Y Tang; K Wang; D Cui; M Li
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of protein chemistry     Volume:  20     ISSN:  0277-8033     ISO Abbreviation:  J. Protein Chem.     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2001-05-01     Completed Date:  2001-10-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8217321     Medline TA:  J Protein Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  59-65     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Peking University, Beijing, China.
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MeSH Terms
Descriptor/Qualifier:
Binding Sites
Concanavalin A / chemistry*,  metabolism*
Crystallography, X-Ray
Models, Molecular
Peptides / chemistry,  metabolism*
Protein Conformation
Chemical
Reg. No./Substance:
0/Peptides; 11028-71-0/Concanavalin A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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