Document Detail


Crystal structure of CATGGCCATG and its implications for A-tract bending models.
MedLine Citation:
PMID:  8464909     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The single-crystal x-ray analysis of orthorhombic CATGGCCATG has revealed a previously unrecognized mode of intrinsic bending in DNA. The decamer shows a smooth bend of 23 degrees over the central four base pairs, caused by preferential stacking interactions at guanine bases. The bend is produced by a roll of base pairs along their long axes, in a direction that compresses the wide major groove of the double helix. This major-groove-compressing bend at GGC, plus the abundant crystallographic evidence that runs of successive adenine bases (A-tracts) are straight and unbent, requires rethinking of the models most commonly invoked to explain A-tract bending. A decade of excellent experimental work involving gel migration experiments, cyclization kinetics, and nucleosome phasing has clearly established that introduction of short A-tracts into a general DNA sequence in synchrony with the natural repeat of the helix leads to bending. But it does not logically and inevitably follow that the actual bending is to be found within these introduced A-tracts or even at junctions with general-sequence B-DNA.
Authors:
D S Goodsell; M L Kopka; D Cascio; R E Dickerson
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  90     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1993 Apr 
Date Detail:
Created Date:  1993-05-04     Completed Date:  1993-05-04     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2930-4     Citation Subset:  IM    
Affiliation:
Molecular Biology Institute, University of California, Los Angeles 90024.
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MeSH Terms
Descriptor/Qualifier:
Base Composition
Base Sequence
Crystallization
Histones / chemistry
Hydrogen Bonding
Models, Molecular
Molecular Sequence Data
Nucleic Acid Conformation*
Oligodeoxyribonucleotides / chemistry*
Protein Binding
X-Ray Diffraction
Grant Support
ID/Acronym/Agency:
GM-31299/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Histones; 0/Oligodeoxyribonucleotides
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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