Document Detail


Crystal structure of the redox-active cofactor dibromothymoquinone bound to circadian clock protein KaiA and structural basis for dibromothymoquinone's ability to prevent stimulation of KaiC phosphorylation by KaiA.
MedLine Citation:
PMID:  23020633     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
KaiA protein that stimulates KaiC phosphorylation in the cyanobacterial circadian clock was recently shown to be destabilized by dibromothymoquinone (DBMIB), thus revealing KaiA as a sensor of the plastoquinone (PQ) redox state and suggesting an indirect control of the clock by light through PQ redox changes. Here we show using X-ray crystallography that several DBMIBs are bound to KaiA dimer. Some binding modes are consistent with oligomerization of N-terminal KaiA pseudoreceiver domains and/or reduced interdomain flexibility. DBMIB bound to the C-terminal KaiA (C-KaiA) domain and limited stimulation of KaiC kinase activity by C-KaiA in the presence of DBMIB demonstrate that the cofactor may weakly inhibit KaiA-KaiC binding.
Authors:
Rekha Pattanayek; Said K Sidiqi; Martin Egli
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-10-05
Journal Detail:
Title:  Biochemistry     Volume:  51     ISSN:  1520-4995     ISO Abbreviation:  Biochemistry     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2013-02-07     Completed Date:  2013-04-01     Revised Date:  2013-10-17    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8050-2     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Vanderbilt University, School of Medicine, Nashville, TN 37232, USA.
Data Bank Information
Bank Name/Acc. No.:
PDB/4G86
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Bacterial Proteins / chemistry*
Circadian Rhythm Signaling Peptides and Proteins / chemistry*
Crystallography, X-Ray
Dibromothymoquinone / chemistry*
Dimerization
Electrophoresis, Polyacrylamide Gel
Molecular Sequence Data
Oxidation-Reduction
Phosphorylation
Protein Conformation
Grant Support
ID/Acronym/Agency:
R01 GM073845/GM/NIGMS NIH HHS; R01 GM073845/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Circadian Rhythm Signaling Peptides and Proteins; 0/KaiA protein, cyanobacteria; 0/KaiC protein, cyanobacteria; 29096-93-3/Dibromothymoquinone
Comments/Corrections

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