| Crystal Structure of Human ASB9-2 and Substrate-Recognition of CKB. | |
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MedLine Citation:
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PMID: 22418839 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Human ankyrin repeat and suppressor of cytokine signaling box protein 9 (hASB9) is a specific substrate-recognition subunit of an elongin C-cullin-SOCS box E3 ubiquitin ligase complex. It recognizes its substrate, brain type creatine kinase (CKB), using the ankyrin repeat domain; and facilitates the polyubiquitination of CKB to mediate proteasomal degradation through the SOCS box domain. HASB9-2 is an isoform of hASB9 that contains one ankyrin repeat domain. In this study, the crystal structure of hASB9-2 is shown at 2.2-Å resolution using molecular replacement. Overall, hASB9-2 forms a slightly curved arch with a characteristic L-shaped cross-section. Amino acid substitution analysis based on docking experiments revealed that His103 and Phe107 in hASB9-2 are essential for binding to CKB. Analysis of truncation mutants demonstrated that the first six ankyrin repeats along with the N-terminal region of hASB9-2 contribute to the interaction with CKB. |
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Authors:
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Xiangwei Fei; Xing Gu; Shilong Fan; Zhenxing Yang; Fan Li; Cheng Zhang; Weimin Gong; Yumin Mao; Chaoneng Ji |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-3-15 |
Journal Detail:
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Title: The protein journal Volume: - ISSN: 1875-8355 ISO Abbreviation: - Publication Date: 2012 Mar |
Date Detail:
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Created Date: 2012-3-15 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101212092 Medline TA: Protein J Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, 200433, People's Republic of China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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