| Cryptotanshinone Suppressed Inflammatory Cytokines Secretion in RAW264.7 Macrophages through Inhibition of the NF-κB and MAPK Signaling Pathways. | |
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MedLine Citation:
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PMID: 20490642 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Cryptotanshinone (CTS), a major constituent extracted from the medicinal herb Salvia miltiorrhiza Bunge, has well-documented antioxidative and anti-inflammatory effects. In the present study, the pharmacological effects and underlying molecular mechanisms of CTS on lipopolysaccharide (LPS)-induced inflammatory responses were investigated. By enzyme-linked immunosorbent assay, we observed that CTS reduced significantly the production of proinflammatory mediators (tumor necrosis factor-α and interleukin-6) induced by LPS in murine macrophage-like RAW264.7 cells. Mechanistically, CTS inhibited markedly the phosphorylation of mitogen-activated protein kinases (MAPKs), including ERK1/2, p38MAPK, and JNK, which are crucially involved in regulation of proinflammatory mediator secretion. Moreover, immunofluorescence and western blot analysis indicated that CTS abolished completely LPS-triggered nuclear factor-κB (NF-κB) activation. Taken together, these data implied that NF-κB and MAPKs might be the potential molecular targets for clarifying the protective effects of CTS on LPS-induced inflammatory cytokine production in macrophages. |
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Authors:
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Shu Tang; Xiao-Yan Shen; He-Qing Huang; Suo-Wen Xu; Yang Yu; Chang-Hua Zhou; Shao-Rui Chen; Kang Le; Yu-Hua Wang; Pei-Qing Liu |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Inflammation Volume: 34 ISSN: 1573-2576 ISO Abbreviation: Inflammation Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-03-04 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7600105 Medline TA: Inflammation Country: United States |
Other Details:
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Languages: eng Pagination: 111-8 Citation Subset: IM |
Affiliation:
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Laboratory of Pharmacology and Toxicology, School of Pharmaceutical Sciences, Sun Yat-sen University, Wai Huan Dong Road 132, Higher Education Mega Center, Guangzhou, 510006, People's Republic of China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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