| Cryptococcus neoformans responds to mannitol by increasing capsule size in vitro and in vivo. | |
| | |
MedLine Citation:
|
PMID: 20070311 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The polysaccharide capsule of the fungus Cryptococcus neoformans is its main virulence factor. In this study, we determined the effects of mannitol and glucose on the capsule and exopolysaccharide production. Growth in mannitol significantly increased capsular volume compared with cultivation in glucose. However, cells grown in glucose concentrations higher than 62.5 mM produced more exopolysaccharide than cells grown in mannitol. The fibre lengths and glycosyl composition of capsular polysaccharide from yeast grown in mannitol was structurally different from that of yeast grown in glucose. Furthermore, mannitol treatment of mice infected intratracheally with C. neoformans resulted in fungal cells with significantly larger capsules and the mice had reduced fungal dissemination to the brain. Our results demonstrate the capacity of carbohydrate source and concentration to modify the expression of a major virulence factor of C. neoformans. These findings may impact the clinical management of cryptococcosis. |
| | |
Authors:
|
Allan Jefferson Guimarães; Susana Frases; Radamés J B Cordero; Leonardo Nimrichter; Arturo Casadevall; Joshua D Nosanchuk |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-01-11 |
Journal Detail:
|
Title: Cellular microbiology Volume: 12 ISSN: 1462-5822 ISO Abbreviation: Cell. Microbiol. Publication Date: 2010 Jun |
Date Detail:
|
Created Date: 2010-06-18 Completed Date: 2010-09-20 Revised Date: 2011-07-28 |
Medline Journal Info:
|
Nlm Unique ID: 100883691 Medline TA: Cell Microbiol Country: England |
Other Details:
|
Languages: eng Pagination: 740-53 Citation Subset: IM |
Affiliation:
|
Department of Medicine, Albert Einstein College of Medicine of Yeshiva University, Bronx, NY, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Brain / microbiology Colony Count, Microbial Cryptococcosis / microbiology* Cryptococcus neoformans / metabolism*, pathogenicity* Glucose / metabolism Lung / microbiology Mannitol / metabolism* Mice Polysaccharides / biosynthesis* Virulence Virulence Factors / biosynthesis* |
| Grant Support | |
ID/Acronym/Agency:
|
AI-51519/AI/NIAID NIH HHS; AI52733/AI/NIAID NIH HHS; D43-TW007129/TW/FIC NIH HHS; R01 AI052733-06A1/AI/NIAID NIH HHS; T32 GM007491/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Polysaccharides; 0/Virulence Factors; 50-99-7/Glucose; 69-65-8/Mannitol |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Helicobacter pylori derived Neutrophil Activating Protein (HP-NAP) increases the lifespan of monocyt...
Next Document: Interleukin-1 receptor phosphorylation activates Rho kinase to disrupt human gastric tight junctiona...