| Cryptococcus gattii in AIDS patients, southern California. | |
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MedLine Citation:
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PMID: 16318719 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cryptococcus isolates from AIDS patients in southern California were characterized by molecular analyses. Pheromone MFalpha1 and MFa1 gene fragments were polymerase chain reaction-amplified with fluorescently labeled primers and analyzed by capillary electrophoresis (CE) on DNA analyzer. CE-fragment-length analyses (CE-FLAs) and CE-single-strand conformation polymorphisms (CE-SSCPs) were used to determine Cryptococcus gattii (Cg), C. neoformans (Cn) varieties neoformans (CnVN) and grubii (CnVG), mating types, and hybrids. Corroborative tests carried out in parallel included growth on specialized media and serotyping with a commercial kit. All 276 clinical strains tested as haploid MATalpha by CE-FLA. CE-SSCP analyses of MFalpha1 showed 219 (79.3%) CnVG, 23 (8.3%) CnVN, and 34 (12.3%) Cg isolates. CE-FLA and CE-SSCP are promising tools for high-throughput screening of Cryptococcus isolates. The high prevalence of Cg was noteworthy, in view of its sporadic reports from AIDS patients in North America and its recent emergence as a primary pathogen on Vancouver Island, Canada. |
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Authors:
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Sudha Chaturvedi; Madhu Dyavaiah; Robert A Larsen; Vishnu Chaturvedi |
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Publication Detail:
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Type: Comparative Study; Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Emerging infectious diseases Volume: 11 ISSN: 1080-6040 ISO Abbreviation: Emerging Infect. Dis. Publication Date: 2005 Nov |
Date Detail:
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Created Date: 2005-12-01 Completed Date: 2006-02-13 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9508155 Medline TA: Emerg Infect Dis Country: United States |
Other Details:
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Languages: eng Pagination: 1686-92 Citation Subset: IM |
Affiliation:
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Mycology Laboratory, Wadsworth Center, 120 New Scotland Avenue, Albany, NY 12208-2002, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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AIDS-Related Opportunistic Infections
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epidemiology,
microbiology* California Cryptococcosis / complications*, epidemiology, microbiology Cryptococcus / classification*, genetics, isolation & purification Culture Media DNA Primers Electrophoresis, Capillary Fungal Proteins / genetics* HIV Infections / complications Humans Mycological Typing Techniques Pheromones / genetics* Polymerase Chain Reaction / methods Polymorphism, Single-Stranded Conformational Reagent Kits, Diagnostic Serotyping |
| Grant Support | |
ID/Acronym/Agency:
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AI 41968/AI/NIAID NIH HHS; AI 48462/AI/NIAID NIH HHS; AI 53732/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/DNA Primers; 0/Fungal Proteins; 0/Pheromones; 0/Reagent Kits, Diagnostic |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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