Document Detail


Cryptochrome 1 from Brassica napus is up-regulated by blue light and controls hypocotyl/stem growth and anthocyanin accumulation.
MedLine Citation:
PMID:  16531484     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cryptochromes are blue/ultraviolet-A light sensing photoreceptors involved in regulating various growth and developmental responses in plants. Investigations on the structure and functions of cryptochromes in plants have been largely confined to Arabidopsis (Arabidopsis thaliana), tomato (Lycopersicon esculentum), and pea (Pisum sativum). We report here the characterization of the cryptochrome 1 gene from Brassica napus (BnCRY1), an oilseed crop, and its functional validation in transgenics. The predicted BnCRY1 protein sequence shows a high degree of sequence identity (94%) to Arabidopsis CRY1. A semiquantitative reverse transcription-polymerase chain reaction and the western-blot analysis revealed that blue light up-regulates its transcript and protein levels in young seedlings. The BnCRY1 promoter harbors conventional light-responsive cis-acting elements, which presumably impart light activation to the GUS (beta-glucuronidase) reporter gene expressed in Arabidopsis. Although the BnCRY1 transcript could be detected in all the tissues examined, its protein was virtually undetectable in mature leaves and the root, indicating a tissue-specific translational control or protein turnover. The antisense-BnCRY1 Brassica transgenic seedlings accumulated negligible levels of CRY1 protein and displayed an elongated hypocotyl when grown under continuous white or blue light (but not under red or far-red light); the accumulation of anthocyanins was also reduced significantly. The adult transformants were also found to be tall when grown under natural light environment in a containment facility without any artificial illumination. These data provide functional evidence for a role of blue light up-regulated cry1 in controlling photomorphogenesis in Brassica species.
Authors:
Mithu Chatterjee; Pooja Sharma; Jitendra P Khurana
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-03-10
Journal Detail:
Title:  Plant physiology     Volume:  141     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-05-10     Completed Date:  2006-07-31     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  61-74     Citation Subset:  IM    
Affiliation:
Interdisciplinary Centre for Plant Genomics, Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi 110021, India.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Anthocyanins / metabolism*
Brassica napus / genetics,  growth & development*,  metabolism
Cryptochromes
Flavoproteins / chemistry,  genetics,  physiology*
Gene Dosage
Gene Expression Regulation, Plant
Glucuronidase / metabolism
Hypocotyl / anatomy & histology,  growth & development,  metabolism
Light*
Molecular Sequence Data
Phylogeny
Plant Leaves / anatomy & histology,  metabolism
Plant Proteins / chemistry,  genetics,  physiology*
Plant Roots / anatomy & histology,  metabolism
Plants, Genetically Modified / metabolism
Polyploidy
Promoter Regions, Genetic
Sequence Alignment
Sequence Analysis, Protein
Up-Regulation*
Chemical
Reg. No./Substance:
0/Anthocyanins; 0/Cryptochromes; 0/Flavoproteins; 0/Plant Proteins; EC 3.2.1.31/Glucuronidase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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