Document Detail


Crypt cell isolation in the small intestine of the mouse.
MedLine Citation:
PMID:  894003     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A successful method has been developed for isolating viable single cell suspensions of intestinal crypt cells from the small bowel of the mouse. The lumen of the intestine was perfused with a 0.2% trypsin solution that dissociated the lining epithelial cells. Crypt cell isolation, which proves to be extremely critical, occurred under optimal mechanical and chemical configurations about 75 min after the beginning of the procedure.
Authors:
V C Kremski; L Varani; C DeSaive; P Miller; C Nicolini
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  25     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  1977 Jul 
Date Detail:
Created Date:  1977-10-28     Completed Date:  1977-10-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  554-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Count
Cell Separation / methods*
Cell Survival
Intestinal Mucosa / cytology*
Intestine, Small / cytology
Mice
Trypsin
Chemical
Reg. No./Substance:
EC 3.4.21.4/Trypsin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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