Document Detail


Crypt Y chromosome fragment resulting from an X;Y translocation in a patient with premature ovarian failure.
MedLine Citation:
PMID:  19524892     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To identify a cryptic Y chromosome fragment that resulted from a X;Y translocation in a patient with premature ovarian failure (POF) and analyze the karyotype-phenotype correlation. DESIGN: Case report. SETTING: A university-based reproductive medicine center. PATIENT(S): A 33-year-old woman with POF. INTERVENTION(S): Karyotyping analysis, comparative genomic hybridization, fluorescence in situ hybridization, and polymerase chain reaction (PCR) analysis for the patient. MAIN OUTCOME MEASURE(S): Karyotype determination of the patient. RESULT(S): The patient was suspected to carry an abnormal X chromosome by traditional cytogenetic analysis. A Y chromosome hybridization signal was found in the patient's genome by comparative genomic hybridization analysis. The fluorescence in situ hybridization result showed that the Y chromosome material resulted from a translocation between Xq and Yq. Using the specific sequence-tagged sites, the breakpoints on the X and Y chromosomes were located at Xq26.3 and Yq11.223, respectively. Combined with chromosome G banding and C banding, the karyotype of the patient was determined as 46,X,der(X)t(X;Y) (q26.3;q11.223). CONCLUSION(S): The advanced molecular cytogenetic techniques are helpful to detect cryptic chromosome aberrancies in patients with POF. This rare case supports that Xq26-q28 is the critical region of POF, and is helpful to analyze the risk of gonadoblastoma in patients with POF with Y chromosomal material.
Authors:
De-Hua Cheng; Yue-Qiu Tan; Yu-Fen Di; Lu-Yun Li; Guang-Xiu Lu
Publication Detail:
Type:  Case Reports; Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-06-13
Journal Detail:
Title:  Fertility and sterility     Volume:  92     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-08-03     Completed Date:  2009-08-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  828.e3-6     Citation Subset:  IM    
Affiliation:
Institute of Reproduction and Stem Cell Engineering, Reproductive and Genetic Hospital of Citic Xiangya, Central South University, Changsha, Peoples Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Adult
Chromosomes, Human, X / genetics*
Chromosomes, Human, Y / genetics*
DNA Fragmentation*
Female
Humans
Ovarian Failure, Premature / diagnosis*,  genetics*
Translocation, Genetic / genetics*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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