Document Detail

Cryotop vitrification of human oocytes results in high survival rate and healthy deliveries.
MedLine Citation:
PMID:  17207335     Owner:  NLM     Status:  MEDLINE    
Vitrification, an ultra-rapid cooling technique, offers a new perspective in attempts to develop an optimal cryopreservation procedure for human oocytes and embryos. To further evaluate this method for human oocytes, 796 mature oocytes (metaphase II) were collected from 120 volunteers. Since Italian legislation allows the fertilization of a maximum of only three oocytes per woman, there were 463 supernumerary oocytes; instead of being discarded, they were vitrified. When, in subsequent cycles, these oocytes were utilized, 328 out of 330 (99.4%) oocytes survived the warming procedure. The fertilization rate, pregnancy rate and implantation rate per embryo were 92.9, 32.5 and 13.2% respectively. Thus, as already reported in the literature, the vitrification procedure seems to be highly effective, safe (since healthy babies have been born) and easy to apply. In situations where embryo cryopreservation is not permitted (as in Italy), there is now good indication for routine application of the method, once further standardization is achieved.
Monica Antinori; Emanuele Licata; Gianluca Dani; Fabrizio Cerusico; Caterina Versaci; Severino Antinori
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Publication Detail:
Type:  Evaluation Studies; Journal Article    
Journal Detail:
Title:  Reproductive biomedicine online     Volume:  14     ISSN:  1472-6483     ISO Abbreviation:  Reprod. Biomed. Online     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2007-01-08     Completed Date:  2007-02-28     Revised Date:  2009-07-20    
Medline Journal Info:
Nlm Unique ID:  101122473     Medline TA:  Reprod Biomed Online     Country:  England    
Other Details:
Languages:  eng     Pagination:  72-9     Citation Subset:  IM    
International Associated Research Institute for Human Reproduction Infertility Unit Day Hospital, Via Timavo No.2, Rome, Italy.
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MeSH Terms
Cell Culture Techniques
Cryopreservation / instrumentation,  methods*,  standards
Embryo Implantation
Pregnancy Rate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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