Document Detail


Cryotolerance of bovine blastocysts is affected by oocyte maturation in media containing palmitic or stearic acid.
MedLine Citation:
PMID:  18992093     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, non-esterified fatty acids (NEFAs) were added during in vitro maturation at concentrations measured previously in follicular fluid (FF) of high-producing dairy cows in a negative energy status to evaluate their subsequent effect on the embryos cryotolerance. Oocytes were matured for 24 h in serum-free media with or without (negative control) the addition of NEFAs dissolved in ethanol or ethanol alone (positive control). Matured oocytes were fertilized and cultured for 7 days in synthetic oviduct fluid medium supplemented with 5% FCS. Embryos that had at least reached the blastocyst stage were vitrified by open pulled straw (OPS) vitrification. Addition of palmitic (C16 : 0) or stearic acid (C18 : 0) during oocyte maturation had significant negative effects on embryo cryotolerance, whereas ethanol or oleic acid (C18 : 1) had no effect. These in vitro results suggest that high NEFA concentrations in FF during a period of negative energy balance in high-yielding dairy cows can have carry-over effects on embryo quality.
Authors:
M A Shehab-El-Deen; J L M R Leroy; D Maes; A Van Soom
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-09-10
Journal Detail:
Title:  Reproduction in domestic animals = Zuchthygiene     Volume:  44     ISSN:  1439-0531     ISO Abbreviation:  Reprod. Domest. Anim.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-20     Completed Date:  2009-03-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9015668     Medline TA:  Reprod Domest Anim     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  140-2     Citation Subset:  IM    
Affiliation:
Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blastocyst / physiology*
Cattle / embryology*
Cells, Cultured
Cryopreservation / methods,  veterinary*
Culture Media
Ethanol / administration & dosage
Female
Fertilization in Vitro / veterinary
Hot Temperature
Male
Oleic Acid / administration & dosage
Oocytes / drug effects,  growth & development*
Palmitic Acid / administration & dosage*
Stearic Acids / administration & dosage*
Chemical
Reg. No./Substance:
0/Culture Media; 0/Stearic Acids; 112-80-1/Oleic Acid; 57-10-3/Palmitic Acid; 57-11-4/stearic acid; 64-17-5/Ethanol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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