| Cryosurgery: ultrastructural changes in pancreas tissue after low temperature exposure. | |
| | |
MedLine Citation:
|
PMID: 17375968 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A number of theoretical and experimental studies, both in vitro and in vivo, have been performed to explain the action of low temperatures on tissue. It is now evident that the thermal parameters used in the past for freezing during cryosurgery were not precise; this may have resulted in the failure of treatment. For the first time, this report describes the early ultrastructural features of pancreatic parenchyma after low temperature exposure, i.e., cryosurgery, in vivo. We demonstrate the effect of freeze-thawing processes using temperatures of various intensities. The cryosurgical response of pancreas parenchyma, i.e., ultrastructural cellular changes in pancreas tissue, was investigated. The electronic microscopic analysis showed that, after local cryodestruction at temperatures of -80 degrees C and -180 degrees C, similar processes occurred within the pancreas tissue in the early postcryosurgical phase -- immediately and up to 24 hours after low temperature exposure on tissue. The exocrine pancreatic cells in the center of the cryozone changed upon thawing. Ultrastructural changes in the exocrine pancreatic cells, where the first signs of dystrophic processes had been noticed, were increased. These ultrastructural changes in the pancreatic cells provide a platform to better understand the mechanisms of damage and the pathogenesis of frostbite after cryosurgery. The properties of the pancreas parenchyma response after low temperature exposure provide important insights into the mechanisms of damage and the cryogenic lesion immediately after thawing in cryosurgery. Our new insights prove on the cell level that suddenly and progressively damaged pancreatic cells in the postcryosurgical zone lead to aseptic cryonecrosis and then to aseptic cryoapoptosis of vital normal tissue. The vascular capillary changes and circulatory stagnation demonstrate the anti-angiogenesis mechanism, which, together with cryoaponecrosis and cryoapoptosis, are some of the main mechanisms of biological tissue injury following the low temperature exposure. |
| | |
Authors:
|
N N Korpan |
Related Documents
:
|
12659918 - Determination of temperature elevation in tissue during the application of the harmonic... 12735568 - Intra-atrial temperatures in radiofrequency endocardial ablation: histologic evaluation... 8134978 - Focal spacing and near-field heating during pulsed high temperature ultrasound therapy. 23733048 - Dual-wavelength photoacoustic technique for monitoring tissue status during thermal tre... 9788908 - Constant-pressure molecular dynamics investigation of cholesterol effects in a dipalmit... 9231398 - Ecophysiological adaptations to dry thermal environments measured in two unrestrained n... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Technology in cancer research & treatment Volume: 6 ISSN: 1533-0346 ISO Abbreviation: Technol. Cancer Res. Treat. Publication Date: 2007 Apr |
Date Detail:
|
Created Date: 2007-03-22 Completed Date: 2007-05-25 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101140941 Medline TA: Technol Cancer Res Treat Country: United States |
Other Details:
|
Languages: eng Pagination: 59-67 Citation Subset: IM |
Affiliation:
|
Vienna International Institute for Cryosurgery, Department of Surgery, Rudolfinerhaus, Billrothstrasse 78, A-1190 Vienna, Austria. Nikolai.Korpan@cryosurgrey.at |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cryosurgery / methods* Dogs Freezing Microscopy, Electron, Transmission Pancreas / surgery*, ultrastructure* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Controlling the growth of "ionic" nanoparticle supracrystals.
Next Document: Cryoablation of renal cancer: variables involved in freezing-induced cell death.