Document Detail


Cryoprotective effects of D-allose on mammalian cells.
MedLine Citation:
PMID:  17645876     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
D-allose, an aldo-hexose, is a rare sugar whose biological functions remain largely unclear. Recently, we demonstrated a novel inhibitory effect of D-allose on production of reactive oxygen species (ROS). Here, we focused on investigating cryoprotective effects of D-allose on cell viability. Mammalian cell lines including OVCAR-3 (human ovarian cancer), HeLa (human cervical cancer), HaCaT (human skin keratinocytes), HDF (human dermal fibroblasts) and NIH3T3 (murine fibroblasts) cells were frozen at -80 degrees C in culture media with various D-allose concentrations. Cells were allowed to recover for 24 h, 1 week or 1 month prior to survival assessment using the trypan blue dye exclusion test, when cell proliferation was evaluated by MTT assay. A beneficial protective role of D-allose on cell survival was found, similar to that of trehalose (disaccharide of glucose), a recognized cryoprotectant. The results suggest that D-allose as a sole additive may provide effective protection for mammalian cells during freezing. Practical studies now need to be performed with D-allose, for example to determine optimal freezing protocols and explore potential for preservation of tissues or organs at non-freezing temperatures.
Authors:
Li Sui; Rika Nomura; Youyi Dong; Fuminori Yamaguchi; Ken Izumori; Masaaki Tokuda
Related Documents :
3793426 - Rheoscopic investigation of red cell deformability in sickle cell anemia.
7288156 - Quantitative assessment of steroid hormone binding sites by thaw-mount autoradiography.
9059316 - Postural vasoconstriction and leg ulceration in homozygous sickle cell disease.
17299996 - Improvement of hepatocyte viability after cryopreservation by supplementation of long-c...
12477936 - Reversible infertility in male mice after oral administration of alkylated imino sugars...
18728126 - The role of thyroid hormone in testicular development and function.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-06-08
Journal Detail:
Title:  Cryobiology     Volume:  55     ISSN:  0011-2240     ISO Abbreviation:  Cryobiology     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-09-03     Completed Date:  2008-01-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  87-92     Citation Subset:  IM    
Affiliation:
Department of Cell Physiology, Faculty of Medicine, Kagawa University, Kagawa, Japan; Kagawa Industry Support Foundation, Kagawa, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line / drug effects*
Cryoprotective Agents / pharmacology*
Glucose / pharmacology*
Hela Cells
Humans
Mice
NIH 3T3 Cells
Chemical
Reg. No./Substance:
0/Cryoprotective Agents; 50-99-7/Glucose; 6038-51-3/allose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Age-dependent changes in the functional expression of two nicotinic receptor subtypes in CA1 stratum...
Next Document:  The evaluation of recombinant hookworm antigens as vaccines in hamsters (Mesocricetus auratus) chall...