Document Detail

Cryoprotection and cryosterilization effects of type I antifreeze protein on E. coli cells.
MedLine Citation:
PMID:  19579654     Owner:  NLM     Status:  MEDLINE    
To establish the effects of type I antifreeze protein (AFP) on E. coli cells, we have focused on the survival rate of the E. coli cells using type I AFP at various concentrations under rapid cooling conditions using liquid N2 at atmospheric or low pressure. The survival rate of E. coli was enhanced by the addition of type I AFP at a concentration of 10 microg/ml, and its value shifted from 0.73% to 2.96%. When the concentration of type I AFP was 100 microg/ml, the cell survival rate markedly decreased to 0.090%. This low survival rate was further decreased (0.022%) by the application of the same freeze-thaw treatment for four times. Also, the effect of type I AFP as a bactericidal agent did not vary according to the varying initial cell densities from 10(4) to 10(8) cells / ml. Furthermore, the effects of using type I AFP at 1.0 MPa with N2 gas under conditions of low pressure and low oxygen tension using a simple device were examined. When the actions of type I AFP as a cryoprotectant were stimulated, the survival rate of the E. coil cells increased to 57.8%. In addition, the bactericidal effect of type I AFP at 100 micro g/ml of protein concentration could also be enhanced. The survival rate using 100 g/ml of type I AFP under low pressure was 0.35% of that using 10 microg/ml under the same conditions. This is the first report on the cryoprotectant and cryosterilization effects of type I AFP of E. coli cells under various conditions.
Hidehisa Kawahara; Sakura Higa; Hiroyuki Tatsukawa; Hitoshi Obata
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biocontrol science     Volume:  14     ISSN:  1342-4815     ISO Abbreviation:  Biocontrol Sci     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-07-07     Completed Date:  2009-07-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9712121     Medline TA:  Biocontrol Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  49-54     Citation Subset:  IM    
Department of Life Science and Biotechnology, Kansai University, 3-3-35 Yamate-cho, Suita, Osaka 564-8680, Japan.
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MeSH Terms
Antifreeze Proteins, Type I / pharmacology*
Atmospheric Pressure
Cryopreservation / methods
Cryoprotective Agents / pharmacology*
Escherichia coli / drug effects*,  physiology
Recombinant Proteins / pharmacology
Sterilization / methods
Reg. No./Substance:
0/Antifreeze Proteins, Type I; 0/Cryoprotective Agents; 0/Recombinant Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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