Document Detail


Cryoprotection by dimethyl sulfoxide and dimethyl sulfone.
MedLine Citation:
PMID:  3816285     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Preservation of cells and tissues at low temperatures requires the presence of effective cryoprotectants with low toxicity to which cells are relatively permeable. Two similar compounds, dimethyl sulfoxide (DMSO) and dimethyl sulfone (DMSO2), exhibit both features for cryoprotectants, yet DMSO is a very effective cryoprotectant while DMSO2 is ineffective. This anomaly was investigated by relating observations on the phase behavior of DMSO and DMSO2 in aqueous solutions to the recovery of human lymphocytes frozen in the presence of these compounds. The lack of cryoprotection in the presence of DMSO2 appears to be due to the precipitation of DMSO2 from the solution at subzero temperatures. The observation of reduced cell recovery after freezing with increasing concentrations of DMSO2 implies that cell damage is related to the amount of solid DMSO2 present. Precipitation of DMSO2 occurs both intra- and extracellularly, but it is argued that intracellular precipitation of DMSO2 is the damaging phenomenon. Cryoprotective compounds are normally selected based on the criteria of low toxicity and permeability to the plasma membrane. An additional condition, solubility, must be included for interpretation of experimental data and for development of effective protocols for cryopreservation.
Authors:
L E McGann; M L Walterson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cryobiology     Volume:  24     ISSN:  0011-2240     ISO Abbreviation:  Cryobiology     Publication Date:  1987 Feb 
Date Detail:
Created Date:  1987-04-14     Completed Date:  1987-04-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  11-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Survival
Dimethyl Sulfoxide*
Freezing
Humans
Lymphocytes / cytology*
Sulfones*
Tissue Preservation / methods*
Chemical
Reg. No./Substance:
0/Sulfones; 67-68-5/Dimethyl Sulfoxide; 67-71-0/dimethyl sulfone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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