| Cryoprotectin protects thylakoids during a freeze-thaw cycle by a mechanism involving stable membrane binding. | |
| | |
MedLine Citation:
|
PMID: 14697731 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Chloroplast thylakoid membranes of higher plants are damaged by freezing both in vivo and in vitro. The resulting inactivation of photosynthetic electron transport has been related to transient membrane rupture, leading to the loss of soluble electron transport proteins and osmotically active solutes from the thylakoid lumen. We have recently purified and sequenced a protein from cold acclimated cabbage, that protects thylakoids from this freeze-thaw damage. The protein belongs to the WAX9 family of nonspecific lipid transfer proteins, but has no detectable lipid transfer activity. Conversely, other transport-active lipid transfer proteins show no cryoprotective activity. We show here that cryoprotectin binds to thylakoid membranes. Both cryoprotective activity and membrane binding were inhibited in the presence of specific sugars, most effectively by Glc-6-S. The binding of cryoprotectin to thylakoids reduced the fluidity of the membrane lipids close to the membrane/solution interface, but not in the hydrophobic core region. Using immobilized liposomes we could show that cryoprotectin was able to bind to pure lipid membranes. |
| | |
Authors:
|
Hany A M Sror; Gilbert Tischendorf; Frank Sieg; Jürgen M Schmitt; Dirk K Hincha |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Cryobiology Volume: 47 ISSN: 0011-2240 ISO Abbreviation: Cryobiology Publication Date: 2003 Dec |
Date Detail:
|
Created Date: 2003-12-30 Completed Date: 2004-09-03 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0006252 Medline TA: Cryobiology Country: United States |
Other Details:
|
Languages: eng Pagination: 191-203 Citation Subset: IM |
Affiliation:
|
Institut für Pflanzenphysiologie und Mikrobiologie, Freie Universität, Königin Luise Str. 12-16, D-14195 Berlin, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Brassica* Cell Membrane / physiology, ultrastructure Cryopreservation* Freeze Fracturing Membrane Lipids / metabolism Plant Leaves / metabolism Spinacia oleracea* Thylakoids / metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Membrane Lipids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Value of the implantable loop recorder for the management of patients with unexplained syncope.
Next Document: Effect of different cryoprotectants and vitrificant solutions on the hatching rate of turbot embryos...