| Cryoprotected Lactobacillus casei: an approach to standardization of microbiological assay of folic acid in serum. | |
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MedLine Citation:
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PMID: 6784966 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Folate-depleted, cryoprotected preparations of Lactobacillus casei are stable for at least eight months at -18 degrees C, capable of reproducible growth and suitable as a ready source of inoculum for measurement of folates in physiological fluids. Cryoprotected microorganisms can be a commercially available laboratory reagent, thus simplifying and further standardizing the microbiological assay of various nutriments. Standard folate growth curves of cryoprotected L. casei, prepared at intervals over eight months, are superimposable, have a low blank, and should eliminate the variations encountered with the continuous passages of microorganisms required for the classic microbiological assays. Serum folate values obtained by use of the cryoprotected L. casei fall into the same diagnostic groups as determined by the classic microbiological assay. |
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Authors:
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N Grossowicz; S Waxman; C Schreiber |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Clinical chemistry Volume: 27 ISSN: 0009-9147 ISO Abbreviation: Clin. Chem. Publication Date: 1981 May |
Date Detail:
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Created Date: 1981-07-09 Completed Date: 1981-07-09 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9421549 Medline TA: Clin Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 745-7 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biological Assay
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methods Cryoprotective Agents Folic Acid / blood*, pharmacology Freezing Glycerol Humans Lactobacillus casei / drug effects, growth & development* |
| Grant Support | |
ID/Acronym/Agency:
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AM-16690-06/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cryoprotective Agents; 56-81-5/Glycerol; 59-30-3/Folic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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