Document Detail


Cryoprotected Lactobacillus casei: an approach to standardization of microbiological assay of folic acid in serum.
MedLine Citation:
PMID:  6784966     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Folate-depleted, cryoprotected preparations of Lactobacillus casei are stable for at least eight months at -18 degrees C, capable of reproducible growth and suitable as a ready source of inoculum for measurement of folates in physiological fluids. Cryoprotected microorganisms can be a commercially available laboratory reagent, thus simplifying and further standardizing the microbiological assay of various nutriments. Standard folate growth curves of cryoprotected L. casei, prepared at intervals over eight months, are superimposable, have a low blank, and should eliminate the variations encountered with the continuous passages of microorganisms required for the classic microbiological assays. Serum folate values obtained by use of the cryoprotected L. casei fall into the same diagnostic groups as determined by the classic microbiological assay.
Authors:
N Grossowicz; S Waxman; C Schreiber
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Clinical chemistry     Volume:  27     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1981 May 
Date Detail:
Created Date:  1981-07-09     Completed Date:  1981-07-09     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  745-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Biological Assay / methods
Cryoprotective Agents
Folic Acid / blood*,  pharmacology
Freezing
Glycerol
Humans
Lactobacillus casei / drug effects,  growth & development*
Grant Support
ID/Acronym/Agency:
AM-16690-06/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
0/Cryoprotective Agents; 56-81-5/Glycerol; 59-30-3/Folic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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