Document Detail


Cryopreserved cells facilitate cell-based drug discovery.
MedLine Citation:
PMID:  17631245     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Advances in detection technologies have enabled an increased use of cell-based functional assays in early drug discovery, in particular for G protein-coupled receptors. Screening assays that use live cells are less prone to generate false positives than assays using lysed cell samples. The use of cryopreserved cells instead of cells that are continuously maintained in culture decreases day-to-day variation, removes passage effects and improves the consistency of cell-based assay results. Cryopreservation techniques uncouple cell culturing from drug-screening activities and allow the use of cells as reagents, just like enzymes in biochemical assays.
Authors:
Guido J R Zaman; Jeroen A D M de Roos; Marion Blomenröhr; Chris J van Koppen; Julia Oosterom
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Publication Detail:
Type:  Journal Article; Review     Date:  2007-06-27
Journal Detail:
Title:  Drug discovery today     Volume:  12     ISSN:  1359-6446     ISO Abbreviation:  Drug Discov. Today     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-07-16     Completed Date:  2008-09-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9604391     Medline TA:  Drug Discov Today     Country:  England    
Other Details:
Languages:  eng     Pagination:  521-6     Citation Subset:  IM    
Affiliation:
Molecular Pharmacology Unit, Organon BioSciences, Oss, The Netherlands. guido.zaman@organon.com
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MeSH Terms
Descriptor/Qualifier:
Biological Assay*
Cell Line
Cryopreservation / methods*
Drug Design
Drug Evaluation, Preclinical / methods*
Humans
Protein Transport
Receptors, G-Protein-Coupled / metabolism
Chemical
Reg. No./Substance:
0/Receptors, G-Protein-Coupled

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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