Document Detail


Cryopreservation and xenotransplantation studies of microencapsulated rat pancreatic islets.
MedLine Citation:
PMID:  11912507     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Islets of Langerhans were isolated from the Sprague Dawley rat pancreas digested by injected collagenase, and purified by Ficoll density gradient centrifugation. In order to make smaller and more uniform microencapsulated islets, we designed a special high-voltage electrostatic microcapsule generator. The effects of operational parameters of the generator on the size and the uniformity of microcapsules were analyzed, such as the voltage, the plunger speed of suspension delivery to the needle tip, the distance between needle tip and solution surface. The optimal parameter combinations for making microcapsules are: 5 kV of voltage, 50 mm/h of the plunger speed, and 20 mm distance. The high-voltage electric system can produce uniform microcapsules with diameters ranging from 0.3 to approximately 0.5 mm, which are smaller and more uniform than those produced by air-jet system. A comparison of the cryopreservation effects between microencapsulated islets and unencapsulated islets showed that the microcapsules can protect the fragile islets from freezing damage, and increase the retrieval rate from 68.5% to 92.6%. Xenotransplantation of the cryopreserved rat islets resulted in the normalization of the metabolic blood glucose of the diabetic mice for 90 days, whereas the unencapsulated islets were easily fragmented and lost during the freezing process. They only reversed hyperglycemia for less than 3-5 days.
Authors:
Bao-Guo Li; Tse-Chao Hua; Hong-De Zhang; Yu-Fei Wang; Guo-Xing Wang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cryo letters     Volume:  23     ISSN:  0143-2044     ISO Abbreviation:  Cryo Letters     Publication Date:    2002 Jan-Feb
Date Detail:
Created Date:  2002-03-25     Completed Date:  2002-05-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9891832     Medline TA:  Cryo Letters     Country:  England    
Other Details:
Languages:  eng     Pagination:  47-54     Citation Subset:  IM    
Affiliation:
Institute of Cryobiological Engineering, Shanghai University of Science and Technology, No.516 Jun Gong Road, Shanghai, 200093, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Glucose / analysis
Cryopreservation / methods*
Diabetes Mellitus, Type 1 / therapy
Drug Compounding / methods*
Insulin / secretion
Islets of Langerhans* / secretion
Islets of Langerhans Transplantation / methods*
Mice
Mice, Inbred BALB C
Models, Animal
Rats
Rats, Sprague-Dawley
Recovery of Function
Transplantation, Heterologous / methods*
Treatment Outcome
Chemical
Reg. No./Substance:
0/Blood Glucose; 11061-68-0/Insulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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