Document Detail

Cryopreservation of transgenic rice suspension cells producing recombinant hCTLA4Ig.
MedLine Citation:
PMID:  17021872     Owner:  NLM     Status:  MEDLINE    
Transgenic suspension cells of Oryza sativa L. cv. Dongjin utilized as a host for producing recombinant human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) were preserved in liquid nitrogen (-196 degrees C) after slow prefreezing in a deep freezer (-70 degrees C). The development of an optimal procedure for long-term storage was investigated by the addition of various concentrations of cryoprotectant mixture and osmoticum in preculture media before cooling. A pre-deep-freezing time of 120 min was the most effective for maintaining cell viability. Compared with mannitol, sorbitol, trehalose, and NaCl under the same osmotic conditions, 0.5 M sucrose was found to be the best osmoticum for preculture media. The cryoprotectant comprising sucrose, glycerol, and dimethylsulfoxide (DMSO) was applied to the precultured cells, and a combination of 1 M sucrose, 1 M glycerol, and 1 M DMSO provided the best result. The viability with this optimized condition was 88% after cryocell-banking for 1 day. The expression of hCTLA4Ig in recovered callus from cryopreservation was also kept stable, and the production level was similar to that observed in noncryopreserved cultures.
Ji-Suk Cho; Seok-Mi Hong; Sung-Yeon Joo; Ji-Seon Yoo; Dong-Il Kim
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-10-05
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  73     ISSN:  0175-7598     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2007-01-11     Completed Date:  2007-05-29     Revised Date:  2013-05-24    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1470-6     Citation Subset:  IM    
Department of Biological Engineering, Inha University, Incheon, 402-751, Republic of Korea.
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MeSH Terms
Cell Survival / drug effects
Cryopreservation / methods*
Immunoconjugates / genetics,  metabolism*
Mannitol / pharmacology
Oryza sativa / cytology*,  genetics
Plants, Genetically Modified
Recombinant Proteins / metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Sodium Chloride / pharmacology
Sorbitol / pharmacology
Sucrose / pharmacology
Time Factors
Trehalose / pharmacology
Reg. No./Substance:
0/Immunoconjugates; 0/Recombinant Proteins; 50-70-4/Sorbitol; 57-50-1/Sucrose; 69-65-8/Mannitol; 7647-14-5/Sodium Chloride; 7D0YB67S97/abatacept; 99-20-7/Trehalose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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