Document Detail

Cryopreservation of protozoan parasites.
MedLine Citation:
PMID:  14969677     Owner:  NLM     Status:  MEDLINE    
Conventional methods for the propagation and preservation of parasites in vivo or in vitro have some limitations, including the need for labor, initial isolation and loss of strains, bacterial, and fungal contamination, and changes in the original biological and metabolic characteristics. All these disadvantages are considerably reduced by cryopreservation. In this study, we examined the effects of various freezing conditions on the survival of several protozoan parasites after cryopreservation. The viability of Entamoeba histolytica was improved by seeding (p < 0.05, chi2 test), while this was not so effective for Trichomonas vaginalis. Of six cryoprotectants examined, dimethyl sulfoxide (Me(2)SO), and glycerol showed the strongest cryoprotective effects. The optimum conditions for using Me(2)SO were a concentration of 10% with no equilibration, and those for glycerol were a concentration of 15% with equilibration for 2h. The optimum cooling rate depended on the parasite species. Trypanosoma brucei gambiense and Leishmania amazonensis were successfully cryopreserved over a wide range of cooling rates, whereas the survival rates of E. histolytica, T. vaginalis, Pentatrichomonas hominis, and Blastocystis hominis were remarkably decreased when frozen at improper rates. Unlike the cooling rate, exposure of the protozoans to a rapid thawing method produced better motility for all parasites.
Yuko Miyake; Panagiotis Karanis; Shoji Uga
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cryobiology     Volume:  48     ISSN:  0011-2240     ISO Abbreviation:  Cryobiology     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-02-18     Completed Date:  2004-10-05     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1-7     Citation Subset:  IM    
Department of Medical Technology, Faculty of Health Science, Kobe University School of Medicine, 7-10-2 Tomogaoka, Suma-ku, Kobe 654-0142, Japan.
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MeSH Terms
Blastocystis hominis / physiology
Cryopreservation / instrumentation,  methods*
Cryoprotective Agents
Entamoeba histolytica / physiology
Eukaryota* / physiology
Leishmania mexicana / physiology
Parasites* / physiology
Trichomonadida / physiology
Trichomonas vaginalis / physiology
Trypanosoma brucei gambiense / physiology
Reg. No./Substance:
0/Cryoprotective Agents

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